Anti-AMPKβ1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y478

Core Product Specifications and Parameters

Biological Background: AMPKβ1 Function and Localization

• AMPKβ1 is the non-catalytic beta-1 subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase.
• It plays a key role in regulating cellular energy metabolism: in response to low ATP, it activates energy-producing pathways and inhibits energy-consuming processes like biosynthesis and cell growth.
• AMPK acts via direct phosphorylation of metabolic enzymes and transcription regulators, exerting both acute and longer-term regulatory effects.
• The beta subunit serves as a scaffold for AMPK complex assembly, its C-terminus bridging the alpha catalytic and gamma regulatory subunits.
• Additionally, AMPKβ1 acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably through indirect activation of myosin.
• Key biological keywords: fatty acid biosynthesis and metabolism, lipid biosynthesis and metabolism, lipoprotein, myristate, phosphoprotein, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen (synthetic peptide within amino acids 50–150) targets an N-terminal region; consider this for detecting total AMPKβ1 levels in your sample system.
• For Western blot, the expected band is at 30 kDa; optimize gel percentage and transfer conditions accordingly.
• In IHC-P, antigen retrieval may be required; validate with appropriate controls.
• For IF/ICC, confirm subcellular localization and choose fixation/permeabilization methods that preserve the epitope.
• The antibody cross-reacts with mouse and rat; verify species-specific reactivity in your experimental setup.
• As a rabbit monoclonal antibody, it typically offers high affinity and specificity; optimize dilution factors for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. This anti-AMPKβ1 antibody supports energy metabolism and AMPK signaling research, and our supply chain can accommodate projects from concept to clinic. Let us bridge your sourcing needs with reliable, scalable solutions.