Anti-AMPKγ1 Polyclonal Antibody for WB, ELISA - P54619

Core Product Specifications and Parameters

Biological Background: AMPKγ1 Function and Localization

• AMPKγ1 (PRKAG1) is the regulatory gamma subunit of the heterotrimeric AMP-activated protein kinase (AMPK), which functions as a master energy sensor in cells.
• It binds AMP, ADP, and ATP through its CBS domains, mediating allosteric regulation of the catalytic alpha subunit: AMP binding promotes kinase activation, while ATP binding promotes deactivation. Related references: PMID:21680840, PMID:24563466
• AMPK activation in response to low cellular energy inhibits ATP-consuming processes such as protein, carbohydrate, and lipid biosynthesis, and also suppresses cell growth and proliferation. Related references: PMID:21680840, PMID:24563466
• Beyond metabolic regulation, AMPK also regulates cellular polarity by remodeling the actin cytoskeleton, likely via indirect activation of myosin. Related references: PMID:21680840, PMID:24563466
• The gamma-1 subunit is subject to alternative splicing, and its activity is further modulated by post-translational modifications including phosphorylation and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 100–200 of human AMPKγ1, a region conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• Predicted molecular weight is approximately 38 kDa; a band near this size should be detected in WB. Consider using lysates from cell lines or tissues known to express AMPK (e.g., liver, muscle) as positive controls.
• For ELISA, the antibody can be used for direct or indirect detection; optimize coating antigen and antibody concentrations empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of validated IVD raw materials, including this AMPKγ1 polyclonal antibody, to support diagnostic manufacturers and researchers in energy metabolism and signaling studies. As a one-stop sourcing partner, we also provide optimized antibody pairs, bulk ancillary reagents, and custom raw-material sourcing for rare targets, ensuring seamless integration from concept to clinical application.