Anti-AMPKα2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P54646

Core Product Specifications and Parameters

Biological Background: AMPKα2 Function and Localization

• AMPKα2 is the catalytic subunit of AMP-activated protein kinase (AMPK), a central energy sensor that regulates cellular metabolism by activating energy-producing pathways and inhibiting energy-consuming processes such as protein, carbohydrate, and lipid biosynthesis. Related references: PMID:17307971 PMID:17712357
• It directly phosphorylates metabolic enzymes, including ACACA, ACACB, and HMGCR, to regulate fatty acid and cholesterol synthesis, and promotes lipolysis via LIPE phosphorylation. Related references: PMID:7959015
• AMPKα2 modulates insulin signaling and glucose uptake by phosphorylating IRS1 and promoting SLC2A4/GLUT4 translocation; it also regulates hepatic glucose homeostasis through CRTC2/TORC2 phosphorylation.
• It governs cell growth and proliferation by phosphorylating mTORC1 pathway components (TSC2, RPTOR, WDR24) and autophagy regulators (ULK1, BECN1, PIK3C3), and inhibits mTORC1 via FNIP1 to promote lysosomal gene expression. Related references: PMID:14651849 PMID:20160076 PMID:21205641 PMID:36732624 PMID:37079666 PMID:28561066
• AMPKα2 acts as a chromatin regulator by phosphorylating histone H2B at Ser-36 and transcription factors such as FOXO3, p53, and CRTC2, thereby influencing energy metabolism gene expression. Related references: PMID:11518699 PMID:11554766 PMID:15866171
• The protein localizes to the cytoplasm, nucleus, and late endosomes, and participates in processes including Wnt signaling, circadian rhythm, and cell migration.
• AMPKα2 belongs to the serine/threonine-protein kinase family, requires magnesium for activity, and is subject to phosphorylation and ubiquitination modifications.
• UniProt keywords highlight roles in autophagy, lipid metabolism, cholesterol biosynthesis, and transcription regulation, with a mature molecular weight of ~62 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 270–343 of human AMPKα2, a region that may be conserved across species; consider verifying sequence identity for your target organism.
• As a rabbit polyclonal antibody, lot-to-lot consistency should be validated by the end user, especially for quantitative applications.
• Recommended applications include WB (endogenous band at ~62 kDa), IF/ICC, and ELISA; optimal dilutions and conditions should be determined empirically.
• Cross-reactivity with mouse and rat makes this antibody suitable for studies in rodent models; however, confirm performance in your specific model system.
• For Western blot, use appropriate gel percentages to resolve the 62 kDa target and include proper positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes by providing one-stop access to IVD raw materials, technical services, and custom sourcing solutions. This AMPKα2 antibody supports research into energy metabolism and cellular signaling pathways. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development from concept to clinic.