Anti-AMPKα2 Rabbit Polyclonal Antibody for Energy Metabolism Research - P54646

Core Product Specifications and Parameters

Biological Background: AMPKα2 Function and Localization

• Acts as the catalytic subunit of AMP-activated protein kinase (AMPK), a master energy sensor that restores cellular energy balance upon ATP depletion.
  Related references: PMID:17307971, PMID:17712357
• Directly phosphorylates and inactivates acetyl-CoA carboxylase (ACACA) and HMG-CoA reductase (HMGCR), thereby inhibiting fatty acid and cholesterol synthesis.
  Related references: PMID:7959015
• Promotes glucose uptake in muscle by facilitating SLC2A4/GLUT4 translocation to the plasma membrane via TBC1D4/AS160 phosphorylation.
• Negatively regulates mTORC1 signaling by phosphorylating TSC2 and RPTOR, linking energy status to cell growth and autophagy.
  Related references: PMID:14651849, PMID:20160076, PMID:21205641
• Modulates transcription by phosphorylating key regulators such as CRTC2, FOXO3, and histone H2B, influencing chromatin structure and gene expression.
  Related references: PMID:11518699, PMID:11554766, PMID:15866171
• Involved in insulin signaling and receptor internalization, impacting glucose homeostasis.
  Related references: PMID:25687571
• Localizes to cytoplasm, nucleus, and late endosomes, reflecting its diverse regulatory roles.
• Plays a role in circadian rhythm by phosphorylating CRY1, and in mitochondrial fragmentation under stress via MTFR1L phosphorylation.
  Related references: PMID:36367943

Experimental Guidance and Technical Tips

• The immunogen spans residues 270–343 within the catalytic kinase domain of human PRKAA2; consider validating antibody specificity in knockout or knockdown models.
• For Western blot, a band around 62 kDa is expected; use whole-cell lysates from human and rat tissues/cell lines based on reported cross-reactivity.
• In immunofluorescence/ICC, permeabilize cells to detect both cytoplasmic and nuclear pools; signal may localize to punctate structures possibly corresponding to late endosomes.
• For ELISA, optimize coating antigen concentration and antibody dilution using the recombinant immunogen as a positive control.
• Inclusion of phosphatase inhibitors in lysis buffers is recommended since AMPKα2 is regulated by phosphorylation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive B2B sourcing platform for IVD raw materials, connecting diagnostic manufacturers and research laboratories with high-quality antibodies and reagents. This AMPKα2 polyclonal antibody supports investigations into energy metabolism, cancer, diabetes, and autophagy-related pathways. Beyond this product, our portfolio includes validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and custom-sourced rare-target raw materials to accelerate your assay development from concept to clinic.