Anti-AMPKa1/AMPKa2 Rabbit pAb for WB, IHC-P, ELISA - Q13131 / P54646

Core Product Specifications and Parameters

Biological Background: AMPKa1/AMPKa2 Function and Localization

• AMPK (AMP-activated protein kinase) is a heterotrimeric energy sensor composed of a catalytic α subunit (PRKAA1/α1 or PRKAA2/α2), a regulatory β subunit, and a γ subunit. The α1 subunit is a 64 kDa serine/threonine kinase critical for cellular energy homeostasis.
• In response to low ATP, AMPK activates catabolic pathways and inhibits anabolic processes such as protein, carbohydrate, and lipid biosynthesis, as well as cell growth and proliferation.
  Related references:
  PMID:17307971, PMID:17712357, PMID:24563466
• AMPK directly phosphorylates transcription regulators (e.g., CRTC2, FOXO3, histone H2B, HDAC5, p53) to modulate gene expression and chromatin structure in response to metabolic stress.
  Related references:
  PMID:11518699, PMID:11554766, PMID:15866171
• It negatively regulates mTORC1 signaling by phosphorylating TSC2, RPTOR, WDR24, and FNIP1, thereby inhibiting cell growth and promoting autophagy under nutrient-poor conditions.
  Related references:
  PMID:14651849, PMID:18439900, PMID:20160076, PMID:36732624, PMID:37079666
• AMPK promotes autophagy by directly phosphorylating and activating ULK1 and WDR45.
  Related references:
  PMID:21205641, PMID:28561066
• AMPK is localized in the cytoplasm and nucleus, and can associate with late endosomes, consistent with its roles in metabolic signaling and trafficking.
• Key post-translational features include phosphorylation (regulating its activity), nucleotide-binding (AMP/ATP), and glycosylation. The enzyme is involved in multiple pathways including lipid/sterol metabolism, autophagy, circadian rhythm, and Wnt signaling.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 13-300) spans a highly conserved region of PRKAA1/PRKAA2, explaining robust cross-reactivity with human, mouse, and rat samples.
• For Western blotting, endogenous AMPKα isoforms are detected as bands around 62–65 kDa; multiple bands may reflect differential splicing or phosphorylation states.
• IHC-P may require antigen retrieval (e.g., citrate buffer pH 6.0) and careful titration; inclusion of appropriate positive and negative tissue controls is recommended.
• ELISA applications can be established using recombinant protein or peptide standards; pair with appropriate secondary antibodies and optimize coating conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-AMPKa1/AMPKa2 product, and bulk ancillary reagents for diagnostic development. With deep expertise in metabolic pathway targets, CamelBio streamlines procurement from concept to clinic, also assisting with rare-target raw material sourcing. For AMPK-related assay projects, we can supply matched detection reagents and technical consultation to accelerate your development timelines.