Anti-AMPKa1/AMPKa2 Monoclonal Antibody for WB, IHC-P, ELISA - Q13131 / P54646

Core Product Specifications and Parameters

Biological Background: AMPKa1/AMPKa2 Function and Localization

• Energy sensor kinase: AMPKα1 (PRKAA1) is the catalytic subunit of AMP-activated protein kinase, acting as a key cellular energy sensor. Upon reduction of intracellular ATP levels, it activates energy-producing pathways and inhibits energy-consuming processes such as protein, carbohydrate, and lipid biosynthesis. Related references: PMID:17307971 PMID:17712357
• Lipid and cholesterol metabolism regulation: It directly phosphorylates and inactivates acetyl-CoA carboxylase (ACACA/ACACB) and HMG-CoA reductase (HMGCR), thereby suppressing fatty acid and cholesterol synthesis. Related references: PMID:17307971 PMID:17712357
• Transcription regulation: AMPKα1 modulates expression of genes involved in energy metabolism by phosphorylating transcription regulators such as CRTC2, FOXO3, HDAC5, MEF2C, and SREBF1. Related references: PMID:11518699 PMID:11554766 PMID:15866171
• mTORC1 and autophagy control: In response to nutrient limitation, AMPKα1 phosphorylates TSC2 and RPTOR to negatively regulate the mTORC1 complex, and phosphorylates ULK1 to initiate autophagy. Related references: PMID:14651849 PMID:18439900 PMID:20160076 PMID:21205641 PMID:28561066
• Subcellular distribution: AMPKα1 is found in the cytoplasm, nucleus, and late endosomes, consistent with its pleiotropic regulatory roles.
• Kinase properties: As a serine/threonine-protein kinase, it binds ATP and Mg²⁺ for catalytic activity and is subject to phosphorylation and ubiquitination, reflecting tight post-translational control.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 251–550 of human AMPKα1 (Q13131); consider this region when designing epitope-specific detection strategies.
• For Western blot (WB), bands are expected at approximately 64 kDa (AMPKα1) and 65 kDa (AMPKα2); validate with appropriate molecular weight markers and positive/negative cell lysates.
• In immunohistochemistry (IHC-P), both cytoplasmic and nuclear staining may be observed; optimize antigen retrieval conditions and antibody titration on tissue microarrays.
• For ELISA applications, verify optimal coating concentration and buffer conditions using recombinant AMPK protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with IVD manufacturers and research laboratories to provide validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents under one roof. This anti-AMPKα1/α2 monoclonal antibody is ideally suited for studies in energy metabolism, autophagy, and cancer signaling, and can be seamlessly integrated into your assay development pipeline. For rare targets or custom sourcing needs, CamelBio’s technical team offers flexible supply solutions and consulting—accelerating your path from concept to clinical application.