Anti-AMPKα1 Rabbit Polyclonal Antibody for WB, ELISA - Q13131

Core Product Specifications and Parameters

Biological Background: AMPKα1 Function and Localization

• Central energy sensor: AMPKα1 is the catalytic subunit of AMP-activated protein kinase, a master energy sensor that responds to low ATP levels by activating energy-producing pathways and inhibiting biosynthesis, thereby maintaining cellular energy homeostasis. Related references: PMID:17307971 PMID:17712357 PMID:24563466
• Lipid metabolism regulation: It phosphorylates and inactivates key lipid metabolic enzymes such as ACACA, ACACB, and HMGCR, suppressing fatty acid and cholesterol synthesis. Additionally, it promotes lipolysis via CHKA phosphorylation. Related references: PMID:34077757
• Transcriptional and chromatin modulation: AMPKα1 regulates gene expression by directly phosphorylating numerous transcription factors and chromatin modifiers (e.g., CRTC2, FOXO3, HDAC5, p53, SREBF1/2, PPARGC1A, and histone H2B), impacting metabolic and stress-responsive transcription. Related references: PMID:11518699 PMID:11554766 PMID:15866171
• mTORC1 and autophagy control: In response to nutrient deprivation, AMPKα1 inhibits the mTORC1 pathway by phosphorylating TSC2 and RPTOR, while also directly phosphorylating GATOR2 subunit WDR24 to suppress glucose-mediated mTORC1 activation. It simultaneously promotes autophagy by activating ULK1. Related references: PMID:14651849 PMID:18439900 PMID:20160076 PMID:36732624 PMID:21205641
• Subcellular localization: AMPKα1 resides in the cytoplasm, nucleus, and late endosomes, enabling its diverse signaling functions in energy sensing, transcriptional regulation, and vesicular trafficking.
• Post-translational modifications and isoforms: As a phosphoprotein and glycoprotein, AMPKα1 is subject to multiple post-translational modifications including phosphorylation and ubiquitination. The PRKAA1 gene generates several isoforms via alternative splicing, potentially expanding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–200 within the N-terminal kinase domain of human AMPKα1, a region highly conserved across species—consistent with reported mouse cross-reactivity.
• For Western blot, an expected band of approximately 64 kDa is predicted. Validate using positive (e.g., AMPKα1-expressing cell lines) and negative controls to confirm specificity.
• In ELISA applications, the polyclonal nature provides multiple epitope recognition. Consider pairing with a monoclonal capture antibody for sandwich ELISA setups.
• As AMPKα1 activity is sensitive to cellular energy status, experimental conditions that elevate AMP (e.g., AICAR treatment or glucose deprivation) may influence detection.
• Always include appropriate controls and optimize antibody concentration for each assay system.

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CamelBio connects diagnostic manufacturers and laboratories with a comprehensive selection of IVD raw materials, including validated antibody pairs, recombinant proteins, and bulk ancillary reagents. This AMPKα1 rabbit polyclonal antibody exemplifies our commitment to providing high-quality tools for metabolic signaling research and diagnostic development. Let our one-stop sourcing expertise streamline your pathway from concept to clinic.