Anti-AMPKα1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13131

Core Product Specifications and Parameters

Biological Background: AMPKα1 Function and Localization

• AMPKα1, encoded by the PRKAA1 gene, is the catalytic alpha-1 subunit of AMP-activated protein kinase (AMPK), also known as ACACA kinase, HMGCR kinase, and tau-protein kinase PRKAA1.
• Serves as a central energy sensor that becomes activated when intracellular ATP levels decline, switching on catabolic pathways and shutting down anabolic processes to restore energy balance. Related references: PMID:17307971 PMID:17712357 PMID:24563466
• Regulates lipid metabolism by directly phosphorylating and inhibiting acetyl-CoA carboxylase (ACACA/ACACB) and HMG-CoA reductase (HMGCR), and by promoting lipolysis through phosphorylation of hormone-sensitive lipase and CHKalpha2. Related references: PMID:34077757
• Controls glucose homeostasis by stimulating GLUT4 translocation to the plasma membrane and phosphorylating insulin signaling components IRS1, PFKFB2, and PFKFB3 (By similarity).
• Modulates cell growth, proliferation, and autophagy by phosphorylating TSC2 and RPTOR to inhibit mTORC1 signaling, and directly activating ULK1 to initiate autophagy. Related references: PMID:14651849 PMID:18439900 PMID:20160076 PMID:21205641 PMID:28561066
• Influences transcription and chromatin structure by phosphorylating transcription regulators such as CRTC2, FOXO3, HDAC5, and p53, as well as histone H2B at Ser36. Related references: PMID:11518699 PMID:11554766 PMID:15866171
• Localizes to the cytoplasm, nucleus, and late endosomes, and is implicated in additional processes including circadian rhythm, Wnt signaling, and host-virus interactions, as indicated by its extensive keyword annotations.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within the kinase domain (aa 381–520), a highly conserved region that likely accounts for the human, mouse, rat cross-reactivity.
• For Western blot (WB), expect a band at ~64 kDa. Use positive control lysates from AMPKα1-expressing cell lines (e.g., HeLa, HEK293).
• For IHC-P and IF/ICC, optimize antigen retrieval and antibody dilution; note that both nuclear and cytoplasmic staining may occur, reflecting AMPKα1 localization.
• The antibody is also suitable for ELISA; consider pairing with a labeled secondary antibody or use in sandwich ELISA with a matched antibody pair.
• As a polyclonal antibody, lot-to-lot consistency may vary; include appropriate controls and re-validate when switching lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to high-quality IVD raw materials, supporting manufacturers from early-stage research to clinical application. For AMPKα1 and related energy metabolism pathways, we offer a comprehensive portfolio including validated antibody pairs, recombinant proteins, and ancillary reagents to streamline assay development. Our expert team assists with rare target sourcing and customized solutions to accelerate your diagnostic kit launch.