Anti-Amphiregulin Monoclonal Antibody for WB, ELISA - P15514

Core Product Specifications and Parameters

Biological Background: Amphiregulin Function and Localization

• Amphiregulin (AREG) is a member of the epidermal growth factor (EGF) family and acts as a ligand for the EGF receptor (EGFR).
• It functions as an autocrine growth factor and a potent mitogen for various cell types, including astrocytes, Schwann cells, and fibroblasts.
• The protein is synthesized as a transmembrane precursor and is localized to the cell membrane.
• Cleavage of the membrane-bound precursor releases the mature soluble form, which contains an EGF-like domain.
• Post-translational modifications include glycosylation and disulfide bond formation, which are critical for its structure and function.
• Alternative names include AR, SDGF, AREGB, and CRDGF (colorectum cell-derived growth factor).
• Human AREG gene (GeneID: 374) encodes a protein with a predicted molecular weight of approximately 28 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature extracellular domain (amino acids 25-252), which is commonly targeted for detecting soluble Amphiregulin in cell supernatants or lysates.
• For Western blot (WB), consider using reducing conditions to resolve the ~28 kDa band; however, note that the protein may appear at various sizes due to glycosylation and processing.
• In ELISA applications, this antibody may be suitable for sandwich pair development when coupled with a matched detection antibody recognizing a distinct epitope.
• Cross-reactivity with rat Amphiregulin has been reported; validate in relevant sample systems if quantitative accuracy is required.
• Always titrate the antibody in your specific assay conditions to determine optimal working dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research labs, offering a comprehensive portfolio from validated antibody pairs to bulk ancillary reagents. Our one-stop platform bridges supply chain gaps, including rare target sourcing like Amphiregulin monoclonal antibodies, to accelerate your assay development and clinical translation. Contact us to customize specifications for your Amphiregulin-targeted applications.