Anti-AMPH Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P49418

Core Product Specifications and Parameters

Biological Background: Amphiphysin Function and Localization

• Amphiphysin (AMPH1, encoded by the AMPH gene) is a protein involved in the regulation of membrane dynamics and the cytoskeleton.
• It participates in mechanisms of regulated exocytosis in synapses and certain endocrine cell types, potentially controlling the properties of the membrane-associated cytoskeleton.
• The protein contains an SH3 domain and coiled-coil regions, which mediate protein-protein interactions.
• It undergoes alternative splicing, producing multiple isoforms; the canonical isoform has a molecular weight of ~76 kDa.
• Subcellularly, amphiphysin localizes to cytoplasmic vesicles, including synaptic vesicle membranes, and associates with the cytoskeleton.
• It is expressed predominantly in neurons, certain endocrine cell types, and spermatocytes, as indicated by tissue specificity data.
• Phosphorylation is a known post-translational modification, and the protein is listed as a phosphoprotein in databases.
• Three-dimensional structural data are available for amphiphysin, highlighting its molecular architecture.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-250 of human amphiphysin, covering the N-terminal region; validate antibody specificity using appropriate positive and negative controls.
• For Western blot, expect a band at approximately 76 kDa; consider using neuronal or endocrine cell lysates as positive samples.
• In immunofluorescence/immunocytochemistry, anticipate a cytoplasmic and vesicular staining pattern; confirm localization with organelle markers if needed.
• For ELISA, optimize coating antigen and antibody concentrations; recombinant amphiphysin protein can serve as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-AMPH antibody supports research in neuroscience and endocrine biology, and CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your assay development.