Anti-AMPD1 Polyclonal Antibody for WB, IF/ICC, ELISA - P23109

Core Product Specifications and Parameters

Biological Background: AMP deaminase 1 Function and Localization

• AMP deaminase 1 (AMPD1), also referred to as myoadenylate deaminase or AMP deaminase isoform M, is encoded by the AMPD1 gene in humans.
• AMPD1 catalyzes the deamination of AMP to IMP, playing a critical role in energy metabolism.
• The enzyme belongs to the hydrolase family and participates in nucleotide metabolism.
• AMPD1 requires zinc as a metal cofactor for its catalytic activity.
• The protein is subject to alternative splicing, resulting in multiple transcript variants.
• It can undergo phosphorylation, indicating that post-translational modification may regulate its function.
• Mutations in AMPD1 are associated with disease variants, linking this enzyme to human genetic disorders.

Experimental Guidance and Technical Tips

• The immunogen was designed using a recombinant protein spanning amino acids 50–260 of human AMPD1 (NP_001166097.1). This N-terminal region may help ensure target specificity.
• For Western blot (WB) analysis, consider using whole-cell or tissue lysates from human, mouse, or rat sources that express AMPD1, such as skeletal muscle. Validate the observed band against the predicted molecular weight of ~86 kDa.
• For immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization conditions for your cell type, and include appropriate controls to verify staining specificity.
• In ELISA applications, use the recombinant immunogen or purified protein as a standard to assess antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing diagnostic manufacturers, laboratories, and research institutes with validated antibody pairs, high-quality monoclonal and polyclonal antibodies, bulk ancillary reagents, and access to rare targets. For energy metabolism and nucleotide metabolism research centered on AMPD1, our Anti-AMPD1 Rabbit Polyclonal Antibody is part of a broader offering that supports your development from concept to clinic, backed by technical and consulting expertise.