Anti-AMOTL1 Rabbit Polyclonal Antibody for WB and ELISA - Q8IY63

Core Product Specifications and Parameters

Biological Background: AMOTL1 Function and Localization

• AMOTL1 (Angiomotin-like protein 1) is a member of the angiomotin family, characterized by coiled-coil domains, and is encoded by the AMOTL1 gene in humans.
• The protein functions as an inhibitor of the canonical Wnt/β-catenin signaling pathway, likely by sequestering β-catenin (CTNNB1) to recycling endosomes, thereby preventing its nuclear accumulation and transcriptional activity.
• AMOTL1 is localized to tight junctions at cell-cell contacts, where it may contribute to epithelial cell polarity and barrier function.
• The AMOTL1 gene undergoes alternative splicing, producing multiple protein isoforms that may have distinct regulatory roles.
• Post-translational modifications include phosphorylation and ubiquitin conjugation (Ubl conjugation), which may modulate its stability, localization, or interaction with signaling partners.
• Genetic variants in AMOTL1 have been associated with disease phenotypes, highlighting its biological significance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 810–900 of human AMOTL1; for Western blotting, expect a band near 107 kDa. Validate the antibody in your target cell or tissue lysates, as post-translational modifications or degradation products may yield additional bands.
• For ELISA, consider using recombinant AMOTL1 protein or peptide fragments spanning the immunogen region as coating antigen; optimize coating and blocking conditions.
• The antibody recognizes human, mouse, and rat AMOTL1; therefore, it can be used across these species, but confirm cross-reactivity in the appropriate biological context.
• Include appropriate positive and negative controls, such as AMOTL1-overexpressing lysates or tissues with known expression (e.g., epithelial cells with tight junctions).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive raw material solutions for IVD manufacturers and research institutions, including validated antibodies, antigen reagents, and ancillary products. This anti-AMOTL1 polyclonal antibody supports investigations into Wnt signaling, tight junction biology, and epithelial cell regulation. Beyond this product, we supply matched antibody pairs, bulk monoclonal and polyclonal antibodies, and custom sourcing for rare targets, all backed by our technical consultation to accelerate your development from concept to clinic.