Anti-AMOT Rabbit Polyclonal Antibody for WB, ELISA - Q4VCS5

Core Product Specifications and Parameters

Biological Background: AMOT Function and Localization

• AMOT, also known as KIAA1071, is a tight junction-associated protein featuring a coiled-coil domain.
• It plays a central role in tight junction maintenance by forming a complex with ARHGAP17, which regulates the uptake of polarity proteins at tight junctions. Related references: PMID:21205866
• AMOT functions as a repressor of YAP1 and WWTR1/TAZ transcriptional activity, likely through regulation of Hippo pathway-mediated phosphorylation of YAP1, leading to its recruitment to tight junctions.
• The protein is involved in endothelial cell migration, tube formation, and the assembly of endothelial cell-cell junctions.
• Localized specifically at tight junctions of cell-cell contacts; expressed in placenta, skeletal muscle, and notably in endothelial cells of capillaries and larger vessels in the placenta.
• Post-translational modifications include phosphorylation and ubiquitin conjugation (Ubl), and the protein undergoes alternative splicing.
• Encoded on human chromosome X, AMOT has a molecular weight of approximately 118 kDa.

Experimental Guidance and Technical Tips

• The immunogen targeting the N-terminal 300 amino acids may confer high specificity; consider validating with full-length protein controls or knockdown lysates.
• For Western blot, a band around 118 kDa is expected; include positive controls such as placental or endothelial cell lysates and appropriate negative controls.
• ELISA can be employed for titer measurement or screening; optimize coating antigen concentration and secondary antibody dilution to achieve the best signal-to-noise ratio.
• Cross-reactivity with rat AMOT suggests utility in rodent models; perform validation in the intended sample system before large-scale application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, including validated polyclonal antibodies like this anti-AMOT antibody, supporting tight junction and Hippo signaling research. We supply optimized antibody pairs, monoclonal/polyclonal reagents, and ancillary materials, helping diagnostic manufacturers and labs accelerate their development from concept to clinic with reliable procurement and technical expertise.