Anti-AMOT Polyclonal Antibody for WB, ELISA - Q4VCS5

Core Product Specifications and Parameters

Biological Background: AMOT Function and Localization

• AMOT (angiomotin) is a key component of tight junctions, forming a complex with ARHGAP17 to regulate the uptake of polarity proteins, thereby maintaining tight junction integrity. Related references: PMID:21205866
• It regulates endothelial cell migration and tube formation, playing a role in angiogenesis and the assembly of endothelial cell-cell junctions.
• AMOT functions as a repressor of YAP1 and WWTR1/TAZ transcription, potentially by modulating Hippo signaling-mediated phosphorylation and tight junction recruitment of YAP1.
• Subcellularly, AMOT localizes to tight junctions at cell-cell contact sites.
• It is expressed in placenta and skeletal muscle, and is specifically found in endothelial cells of capillaries and larger vessels in the placenta.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond formation), which may regulate its function and stability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the C-terminal region (aa 1000–1084) of human AMOT; consider verifying isoform specificity if multiple variants are expected.
• For Western blot, a band around 118 kDa is anticipated; validate detection of endogenous target in relevant cell or tissue lysates (e.g., placenta, endothelial cells).
• ELISA performance may vary; optimize coating antigen and antibody dilution using appropriate positive and negative controls.
• Cross-reactivity with mouse and rat AMOT is reported, but sequence alignment is recommended to confirm epitope conservation before cross-species applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing one-stop access to high-quality IVD raw materials, including validated antibody pairs and polyclonal antibodies like this AMOT reagent. Our expertise extends to bulk ancillary reagents and rare target sourcing, ensuring a reliable supply chain for tight junction-related research and assay development. Partner with us to bridge your concept-to-clinic needs efficiently.