Anti-AMHR2 Monoclonal Antibody for WB, ELISA - Q16671

Core Product Specifications and Parameters

Biological Background: AMHR2 Function and Localization

• AMHR2 encodes the anti-Muellerian hormone type-2 receptor (also known as AMH type II receptor, MIS type II receptor; aliases MISRII, MRII), a transmembrane serine/threonine kinase.
• The protein is a key component of the AMH signaling pathway, mediating Müllerian duct regression in male sexual differentiation.
• Upon AMH binding, two type II receptors and two type I receptors form a heterotetrameric complex, where type II receptors phosphorylate and activate type I receptors, which then phosphorylate SMAD transcriptional regulators to modulate gene expression.
• AMHR2 is localized to the plasma membrane (membrane-bound receptor).
• The receptor contains intrinsic kinase activity, requiring ATP and divalent cations (Mg²⁺/Mn²⁺) for function, and undergoes post-translational modifications including glycosylation and disulfide bond formation.
• Alternative splicing generates multiple isoforms; mutations in AMHR2 are linked to persistent Müllerian duct syndrome (PMDS), a form of pseudohermaphroditism.

Experimental Guidance and Technical Tips

• The immunogen was designed using a synthetic peptide from the N-terminal extracellular domain (aa 50-150) of human AMHR2, which may confer specificity for extracellular epitopes in both native and denaturing conditions.
• This antibody is validated for WB and ELISA; for WB, a band around 52-63 kDa (likely corresponding to glycosylated or alternatively spliced isoforms) may be observed.
• For ELISA, consider coating with recombinant AMHR2 protein or peptide to establish standard curves.
• Since cross-reactivity is confirmed only for human, validate species specificity if used with other samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers in reproductive biology with one-stop access to IVD raw materials. Beyond this AMHR2 antibody, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare receptor targets. Contact us to streamline your assay development from concept to clinic.