Anti-AMH Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P03971

Core Product Specifications and Parameters

Biological Background: AMH Function and Localization

• AMH is a secreted glycoprotein belonging to the TGF-beta superfamily, with a molecular weight of approximately 59 kDa.
• It signals through its specific type-II receptor AMHR2, which heterodimerizes with type-I receptors ACVR1 and BMPR1A, leading to SMAD-mediated transcriptional regulation (PubMed:20861221, PubMed:34155118).
  Related references: PMID:20861221 PMID:34155118
• In the male fetus, Sertoli cell-derived AMH induces Muellerian duct regression, a key event in normal male reproductive tract development (PubMed:34155118, PubMed:3754790, PubMed:8469238).
  Related references: PMID:34155118 PMID:3754790 PMID:8469238
• In females, AMH is produced by ovarian granulosa cells and inhibits primordial follicle transition while reducing FSH sensitivity in growing follicles (PubMed:14742691).
  Related references: PMID:14742691
• AMH is also implicated in Leydig cell differentiation and function (By similarity).
• The hormone is secreted into the circulation and can be detected in serum.
• AMH is linked to disorders of sexual development, as indicated by disease variant and pseudohermaphroditism keywords.
• Post-translational modifications include disulfide bond formation and glycosylation, typical of secreted growth factors.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 360-560) of human AMH, which includes part of the mature active hormone. Consider this when designing detection strategies.
• For Western blot (WB), expect a band at approximately 59 kDa under reducing conditions. Validate the antibody with positive control lysates from testis or ovarian tissue, where AMH is expressed.
• For immunofluorescence (IF-P) and immunohistochemistry (IHC-P), AMH is localized to Sertoli cells in fetal testis and granulosa cells in ovarian follicles. Optimize antigen retrieval and use appropriate isotype controls.
• In ELISA applications, pairing with a capture antibody targeting a different epitope may improve sensitivity. Validate the assay with recombinant AMH protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, supporting diagnostic manufacturers and research institutions from concept to clinic. Our catalog includes validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, enabling seamless integration into your assay development. For rare or specialized targets like AMH, CamelBio offers sourcing expertise to accelerate your reproductive health and developmental biology projects.