Anti-AMFR Rabbit Polyclonal Antibody for WB and ELISA - Q9UKV5

Core Product Specifications and Parameters

Biological Background: E3 ubiquitin-protein ligase AMFR Function and Localization

• AMFR (gp78/RNF45) is a multi-pass transmembrane E3 ubiquitin-protein ligase that mediates polyubiquitination of lysine and cysteine residues on target proteins such as CD3D, CYP3A4, CFTR, INSIG1, SOAT2 and APOB, directing them for proteasomal degradation. Related references: PMID:10456327 PMID:11724934 PMID:12670940
• It functions as a core component of the VCP/p97-AMFR/gp78 complex, which participates in the final step of endoplasmic reticulum-associated degradation (ERAD). Related references: PMID:10456327 PMID:11724934 PMID:19103148
• Plays a critical role in sterol-accelerated ERAD of HMGCR by interacting with the HMGCR-INSIG1 complex and with AUP1 to facilitate ubiquitination by UBE2G2 and RNF139, leading to subsequent degradation. Related references: PMID:16168377 PMID:22143767 PMID:23223569
• Catalyzes ubiquitination on cysteine residues: together with INSIG1, mediates polyubiquitination of SOAT2/ACAT2 at Cys-277 for degradation when lipid levels are low, and also ubiquitinates INSIG1/2 for degradation. Related references: PMID:28604676 PMID:17043353 PMID:31953408
• Acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation during ERAD, including ubiquitination of UBL4A. Related references: PMID:21636303
• Serves as a receptor for GPI-anchored autocrine motility factor (AMF), mediating tumor invasion and metastasis. Related references: PMID:10456327
• Negatively regulates the canonical Wnt signaling pathway in lymphocytes by promoting ubiquitin-mediated degradation of CTNNB1, FZD6 and LRP6. Related references: PMID:31073040
• Localizes to the endoplasmic reticulum membrane and is widely expressed across tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 384–643 of human AMFR. Users may consider validating detection in their specific assay system and sample type.
• For Western blot, expect a band near 73 kDa; include appropriate positive controls such as human cell lysates.
• For ELISA, pairing with another antibody targeting a distinct epitope may enhance sensitivity and assay performance.
• As a polyclonal antibody, lot-to-lot consistency should be evaluated for critical applications.
• Cross-reactivity is reported for human; verify reactivity with your target species if different.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. The AMFR antibody is well-suited for research into ER-associated degradation, ubiquitination pathways, and cancer biology. We support diagnostic manufacturers with one-stop procurement solutions, from concept to clinic, including rare target sourcing and technical consulting.