Anti-AMD1 Polyclonal Antibody for WB, ELISA - P17707

Core Product Specifications and Parameters

Biological Background: AMD1 Function and Localization

• AMD1 (also known as SAMDC, ADOMETDC) encodes S-adenosylmethionine decarboxylase proenzyme, a key enzyme in the polyamine biosynthesis pathway.
• The enzyme catalyzes the decarboxylation of S-adenosylmethionine to produce decarboxylated S-adenosylmethionine, a critical step for the synthesis of spermidine and spermine.
• The active enzyme is generated by autocatalytic cleavage of the proenzyme, producing a pyruvoyl group from a serine residue which is essential for catalytic activity.
• AMD1 is implicated in the maintenance and self-renewal of embryonic stem cells through the regulation of intracellular spermine levels.
• The protein is subject to phosphorylation and alternative splicing, generating multiple isoforms that may differ in activity or regulation.
• Molecular weight of the unprocessed proenzyme is approximately 38.3 kDa (calculated from amino acid sequence).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a large internal region of human AMD1 (aa 75–334), which may recognize multiple isoforms or processed forms; consider verifying the detected band sizes in your sample system.
• For Western blot analysis, the predicted molecular weight is around 38 kDa, but autocatalytic processing can yield smaller fragments; optimize denaturing conditions to prevent artefactual bands.
• The antibody exhibits cross-reactivity with rodent orthologs, making it suitable for translational studies involving mouse or rat tissues.
• Because AMD1 is a low-abundance enzyme, signal enhancement techniques may be needed; validate the antibody in ELISA and WB with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bundles diagnostic manufacturers, laboratories, and research institutes with a consolidated supply chain for IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For AMD1/polyamine metabolism research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target custom sourcing. Whether you need off-the-shelf reagents or bespoke development, CamelBio bridges the gap between discovery and diagnostic application.