Anti-AMBRA1 Rabbit Polyclonal Antibody for WB and ELISA - Q9C0C7

Core Product Specifications and Parameters

Biological Background: AMBRA1 Function and Localization

• AMBRA1 is the substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, mediating polyubiquitination of target proteins involved in cell cycle progression and autophagy. Related references: PMID:20921139, PMID:23524951, PMID:24587252
• Serves as an upstream master regulator of the G1/S transition by recognizing phosphorylated cyclin-D (CCND1, CCND2, CCND3) and targeting them for ubiquitin-dependent degradation, thereby acting as a tumor suppressor. Related references: PMID:33854232, PMID:33854235, PMID:33854239
• Acts as a key autophagy regulator; upon autophagy induction, it is released from the cytoskeleton to mediate 'Lys-63'-linked ubiquitination of BECN1 and ULK1, enhancing PIK3C3 activity and ULK1 stability to promote autophagosome formation. Related references: PMID:20921139, PMID:23524951
• Promotes mitophagy by interacting with PRKN and LC3 family proteins to target damaged mitochondria to autophagosomes, and acts as a cofactor for HUWE1-mediated ubiquitination of MFN2. Related references: PMID:21753002, PMID:25215947, PMID:30217973
• Independently of its E3 ligase function, AMBRA1 regulates MYC and FOXO3 stability through interactions with protein phosphatase 2A (PPP2CA), leading to MYC degradation and FOXO3 stabilization, thus impacting cell proliferation and regulatory T-cell differentiation. Related references: PMID:25438055, PMID:25803737, PMID:30513302
• Subcellular localization includes cytoplasm, cytoskeleton, endoplasmic reticulum, autophagosomes, mitochondria, nucleus, and focal adhesions, reflecting its multifaceted roles in cellular trafficking and signaling.
• Post-translationally modified by methylation and phosphorylation, and contains WD repeats; it is also involved in 'Lys-63'-linked polyubiquitin chain assembly, critical for signal transduction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 961–1208 of human AMBRA1; thus the antibody likely recognizes epitopes within this region. In Western blot, expect a band at approximately 143 kDa. Consider optimizing blocking and washing conditions to minimize background.
• Confirmed mouse cross-reactivity makes this antibody suitable for mouse model studies; however, validate detection in the specific tissue or cell type of interest to ensure specificity and sensitivity.
• For ELISA applications, pre-coating conditions and detection systems should be empirically optimized; consider using recombinant AMBRA1 protein or cell lysates as coating antigens.
• Due to alternative splicing, multiple AMBRA1 isoforms may exist in certain cell types; verify the detected band pattern against known controls in your experimental system.
• This polyclonal antibody may be used for immunoprecipitation or immunofluorescence with additional validation; non-specific binding to other WD repeat-containing proteins is unlikely but should be ruled out by appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive, one-stop sourcing solution for IVD raw materials, technical services, and regulatory consulting—from concept to clinic. This anti-AMBRA1 polyclonal antibody supports research into autophagy, cell cycle regulation, and tumor suppression. Beyond this product, our extensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets—all backed by our dedication to accelerating your diagnostic development.