Anti-Ambra1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9C0C7

Core Product Specifications and Parameters

Biological Background: Ambra1 Function and Localization

• Acts as a substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase complex, mediating polyubiquitination of target proteins such as BECN1, cyclin-D, ELOC, and ULK1 (PubMed:20921139, PubMed:23524951, PubMed:24587252). Related references: PMID:20921139 PMID:23524951 PMID:24587252
• Functions as an upstream master regulator of the G1/S transition by recognizing phosphorylated cyclin-D (CCND1/2/3) and promoting its ubiquitination and degradation, thereby acting as a tumor suppressor (PubMed:33854232, PubMed:33854235, PubMed:33854239). Related references: PMID:33854232 PMID:33854235 PMID:33854239
• Regulates MYC stability independently of the DCX complex by recruiting protein phosphatase 2A (PPP2CA) to dephosphorylate and promote degradation of MYC (PubMed:25438055, PubMed:25803737). Related references: PMID:25438055 PMID:25803737
• Key regulator of autophagy: upon autophagy induction, AMBRA1 is released from dynein motors and mediates 'Lys-63'-linked ubiquitination of BECN1 and ULK1 to enhance PIK3C3 activity and ULK1 stability, promoting autophagosome nucleation (PubMed:20921139, PubMed:23524951, PubMed:31123703). Related references: PMID:20921139 PMID:23524951 PMID:31123703
• Involved in mitophagy by interacting with PRKN and LC3 proteins to recruit damaged mitochondria, and by acting as a cofactor for HUWE1-mediated ubiquitination of MFN2 (PubMed:21753002, PubMed:25215947, PubMed:30217973). Related references: PMID:21753002 PMID:25215947 PMID:30217973
• Promotes regulatory T-cell differentiation by dephosphorylating and stabilizing FOXO3 via interaction with PPP2CA (PubMed:30513302). Related references: PMID:30513302
• Localizes to multiple subcellular compartments including cytosol, cytoskeleton, autophagosomes, mitochondria, nucleus, and focal adhesions, consistent with its diverse regulatory roles.
• Contains WD repeats and undergoes phosphorylation and methylation; alternative splicing yields multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1109–1208 of human Ambra1, a region within the C-terminal WD repeat domain; consider sequence homology when predicting cross-reactivity across species.
• For Western blot analysis, the predicted molecular weight is approximately 143 kDa; thorough blocking and appropriate loading controls are recommended for clear detection.
• This antibody is listed for applications including WB, IHC-P, IF/ICC, and ELISA; optimal dilutions and incubation conditions should be empirically validated in the end-user’s specific sample system.
• Cross-reactivity with mouse and rat has been indicated; verify performance in the intended model species and tissue type before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Ambra1 polyclonal antibody aids autophagy, cell cycle, and tumor suppression research and is part of our extensive antibody portfolio. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.