Anti-AMBP Rabbit Polyclonal Antibody for WB, ELISA - P02760

Core Product Specifications and Parameters

Biological Background: AMBP Function and Localization

• The AMBP gene encodes a precursor protein that is cleaved into two functional chains: alpha-1-microglobulin (protein HC/A1M) with antioxidant and tissue repair activities, and bikunin, a kunitz-type serine protease inhibitor.
• Alpha-1-microglobulin (A1M) exhibits reductase, heme-binding, and radical-scavenging activities. It reduces extracellular methemoglobin, binds and degrades free heme to prevent hemolysis, and protects against oxidative damage to low-density lipoprotein particles and extracellular matrix structures. Related references: PMID:11877257 PMID:15683711 PMID:22096585 PMID:32092412 PMID:25698971
• Within mitochondria, A1M associates with complex I of the respiratory chain, maintaining redox homeostasis by scavenging free radicals and reducing cytochrome c from Fe3+ to Fe2+. Related references: PMID:23157686 PMID:32823731
• Bikunin functions as a Kunitz-type serine protease inhibitor, effectively inhibiting kallikrein, granzyme K, plasmin, factor Xa, trypsin, chymase, and tryptase. It plays a role in modulating airway inflammation and cytotoxic responses. Related references: PMID:10480954 PMID:15917224 PMID:16873769
• Bikunin is also a structural component of the extracellular matrix, contributing to extracellular space remodeling and cell adhesion. It forms part of the inter-alpha-trypsin inhibitor complex, facilitating heavy chain transfer to hyaluronan, and inhibits calcium oxalate crystallization. Related references: PMID:20463016 PMID:25301953 PMID:16873769 PMID:7676539
• AMBP is widely expressed by the liver and secreted into plasma, urine, and cerebrospinal fluid. It is also detected in red blood cells, placental tissue, keratinocytes, dermis, and airway epithelium. Subcellularly, it localizes to the extracellular space, endoplasmic reticulum, cytoplasm, cell membrane, nucleus membrane, and mitochondrial inner membrane.

Experimental Guidance and Technical Tips

• The immunogen used spans amino acids 20-202 of human AMBP, covering the alpha-1-microglobulin (A1M) chain. The antibody is expected to recognize A1M and may also detect the full-length precursor; consider this when interpreting bands in Western blot.
• In WB, the full-length AMBP has a predicted molecular weight of ~39 kDa; however, processed chains or glycosylated forms may appear at different sizes. Use appropriate molecular weight markers and consider reducing vs. non-reducing conditions.
• For ELISA applications, this polyclonal antibody can be used as a detection reagent. If a sandwich ELISA format is desired, pairing with a monoclonal capture antibody specific to a different epitope may improve assay specificity.
• The antibody cross-reacts with mouse AMBP, indicating potential epitope conservation. Validation in mouse-derived samples is recommended before use in mouse experimental systems.
• Due to the presence of disulfide bonds in bikunin, non-reducing conditions may be necessary to preserve epitope recognition if targeting the bikunin chain.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with convenient access to high-quality IVD raw materials. This Anti-AMBP Rabbit Polyclonal Antibody supports research into oxidative stress, protease inhibition, and extracellular matrix biology. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare-target raw materials to help streamline your assay development from concept to clinic.