Anti-AMACR Rabbit Polyclonal Antibody for WB, IF, IHC, ELISA - Q9UHK6

Core Product Specifications and Parameters

Biological Background: Alpha-methylacyl-CoA racemase Function and Localization

• Alpha-methylacyl-CoA racemase (AMACR), also termed 2-methylacyl-CoA racemase, is an enzyme with dual localization in peroxisomes and mitochondria.
• It catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters, essential for the degradation of branched-chain fatty acids. Substrates include pristanoyl-CoA, trihydroxycoprostanoyl-CoA (intermediate in bile acid synthesis), and the drug ibuprofen. Related references: PMID:7649182, PMID:10655068, PMID:11060359
• AMACR acts exclusively on coenzyme A thioesters and does not process free fatty acids or 3-methyl-branched/linear-chain acyl-CoAs.
• The enzyme is encoded by the AMACR gene, which undergoes alternative splicing to produce multiple protein isoforms.
• Post-translational modifications include acetylation, which may regulate its activity or stability.
• Defects in AMACR are associated with intrahepatic cholestasis and other metabolic conditions, highlighting its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant protein corresponding to amino acids 1–138 of human AMACR (NP_055139.4). This N-terminal region is likely to generate antibodies recognizing multiple isoforms.
• For Western blot (WB), a band around 42 kDa is expected based on the predicted molecular weight.
• Immunofluorescence (IF) and immunohistochemistry (IHC) can be employed to visualize AMACR localization in cells and tissues; consider including appropriate organelle markers to confirm peroxisomal and mitochondrial distribution.
• Given the high sequence conservation among human, mouse, and rat AMACR, this antibody is predicted to detect the protein in these species; however, validation in the relevant species and sample type is recommended.
• The antibody may be suitable for ELISA applications; however, appropriate assay optimization and controls are necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to essential IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For AMACR-focused applications in peroxisomal metabolism, bile acid disorders, or cancer biomarker studies, we can source tailored raw materials to accelerate your assay development from concept to clinic. Contact our team to discuss custom procurement needs.