Anti-ALX4 Rabbit Polyclonal Antibody for WB, ELISA - Q9H161

Core Product Specifications and Parameters

Biological Background: ALX4 Function and Localization

• ALX4 (aristaless-like homeobox 4) is a homeodomain-containing transcription factor and a member of the ALX family of homeobox proteins, which are critical regulators of developmental processes.
• It functions as a DNA-binding transcriptional activator, orchestrating the expression of genes essential for skull morphogenesis, limb development, and craniofacial patterning.
• The protein is predominantly localized in the nucleus, where it engages with chromatin to modulate target gene transcription.
• Post-translational modifications, particularly phosphorylation, are documented for ALX4 and may influence its transcriptional activity, stability, or interactions with co-factors.
• ALX4 exhibits a restricted tissue expression profile, with significant expression in parietal bone and other osteogenic tissues, aligning with its role in skeletal development.
• Mutations in the ALX4 gene are linked to human congenital disorders such as craniosynostosis, underscoring its importance in proper skull formation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–220) of human ALX4; this design may yield antibodies that recognize the full-length protein and possibly conserved domains across species, as supported by the observed cross-reactivity with mouse and rat.
• For Western blot (WB), a band at approximately 44 kDa is anticipated; use lysates from bone-related cell lines (e.g., osteoblasts) or tissues where ALX4 is expressed. Include proper positive and negative controls.
• In ELISA, the antibody can be used for direct or indirect detection of ALX4 protein or peptide fragments; optimize coating and detection conditions empirically.
• As a polyclonal antibody, it may exhibit cross-reactivity to other homeobox proteins; if specificity is crucial, consider pre-adsorption or use in combination with other validation methods (e.g., siRNA knockdown).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material sourcing partner, providing diagnostic manufacturers and research laboratories with reliable access to antibodies, antigens, and ancillary reagents. This anti-ALX4 polyclonal antibody supports studies in developmental biology, skeletal disorders, and craniofacial anomalies, and we offer scalable supplies of validated antibodies, recombinant proteins, and custom solutions to streamline your assay development and production needs.