Anti-ALS2CR1 Rabbit Monoclonal Antibody for WB, ELISA - Q9GZT8

Core Product Specifications and Parameters

Biological Background: ALS2CR1 (NIF3L1) Function and Localization

• ALS2CR1, also known as NIF3-like protein 1 (NIF3L1), CALS-7, or MDS015, is a 42 kDa protein encoded by the NIF3L1 gene in humans (GeneID: 60491).
• It may function as a transcriptional corepressor by interacting with the COP9 signalosome subunit COPS2, thereby negatively regulating the expression of genes involved in neuronal differentiation.
• The protein localizes to both the cytoplasm and nucleus, consistent with a role in shuttling between compartments to mediate transcriptional regulation.
• ALS2CR1 undergoes post-translational modifications including phosphorylation and acetylation, which may modulate its activity or interactions.
• Alternative splicing of the NIF3L1 gene generates multiple transcript variants, though the functional significance of these isoforms remains to be fully characterized.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 250–350 of human ALS2CR1; the antibody may recognize the endogenous full-length protein in human samples.
• For Western blot, the predicted molecular weight is approximately 42 kDa; consider using a nuclear and cytoplasmic fraction preparation to confirm localization.
• For ELISA, the synthetic peptide can serve as a positive control; titration experiments are recommended to determine optimal antibody concentration.
• Negative control experiments (e.g., siRNA knockdown or knockout cell lines) should be used to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This ALS2CR1 rabbit monoclonal antibody fits into neuronal differentiation and gene regulation research applications. For rare target sourcing or customized raw-material needs, CamelBio’s integrated supply chain can help bridge the gap from concept to clinic.