Anti-ALPPL2 Rabbit Polyclonal Antibody for WB, ELISA - P10696

Core Product Specifications and Parameters

Biological Background: ALPPL2 Function and Localization

• ALPPL2, also known as germ cell alkaline phosphatase (GCAP), placental alkaline phosphatase-like (PLAP-like), or alkaline phosphatase Nagao isozyme, is a member of the alkaline phosphatase family.
• It functions as a hydrolase that can non-specifically dephosphorylate a variety of phosphate compounds, playing a role in phosphate metabolism.
• The protein is anchored to the cell membrane via a GPI-linkage, and its sequence includes a signal peptide, indicating secretion and membrane targeting.
• Tissue expression is highly restricted: trace amounts are found in testis and thymus, but levels are elevated in germ cell tumors, making it a potential tumor marker.
• Key structural features include multiple disulfide bonds, glycosylation sites, and a metal-binding domain that coordinates calcium, zinc, and magnesium ions essential for catalytic activity.
• The mature protein has a calculated molecular weight of approximately 57 kDa, consistent with the observed band in western blotting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 20–280), suggesting the antibody is likely to recognize both native and denatured forms of ALPPL2, making it suitable for WB under reducing and non-reducing conditions.
• For ELISA, consider using recombinant ALPPL2 protein as a coating antigen, or cell lysates from ALPPL2-expressing lines (e.g., germ cell tumor-derived cells).
• Given the cross-reactivity with mouse, validation in mouse testis or thymus tissue lysates is recommended if applying to in vivo models.
• This polyclonal antibody may detect multiple bands around 57 kDa due to glycosylation heterogeneity; treat samples with deglycosylation enzymes if a single band is desired.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a complete sourcing solution for IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This anti-ALPPL2 antibody is well-suited for developers creating diagnostic assays targeting germ cell tumors or alkaline phosphatase biomarkers. Leveraging our global network, we can rapidly supply research-grade and production-grade materials, along with technical consultation, to accelerate your project from concept to clinic.