Anti-ALPI (IAP) Polyclonal Antibody for WB, ELISA - P09923

Core Product Specifications and Parameters

Biological Background: ALPI Function and Localization

• ALPI (intestinal-type alkaline phosphatase) is a membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at alkaline pH.
• It is anchored to the outer leaflet of the cell membrane via a glycosylphosphatidylinositol (GPI) anchor, enabling its function at the cell surface.
• The enzyme’s catalytic activity depends on divalent metal ions, including zinc, magnesium, and calcium, which are coordinated within the active site.
• Post-translational modifications include disulfide bond formation and N-linked glycosylation, which are critical for proper folding and stability.
• ALPI is the intestinal isozyme of the alkaline phosphatase family and may participate in gut mucosal defense by dephosphorylating bacterial endotoxins.
• It is classified as a hydrolase within the alkaline phosphatase family and is widely used as a research tool in studying intestinal biology and phosphate metabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment covering amino acids 20–200 of human ALPI; the resulting polyclonal antibody recognizes native and denatured forms, as indicated by validated Western blot and ELISA applications.
• For Western blot, a band around 57 kDa is expected; however, tissue-specific glycosylation may cause slight shifts in apparent molecular weight.
• Given cross-reactivity with human and mouse, consider this antibody for studies in either species, but always validate in the relevant sample system with appropriate controls.
• Optimize blocking and antibody dilution empirically for each application, and include a positive control known to express ALPI (e.g., intestinal tissue lysates).

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio streamlines your procurement of high-quality antibodies like this anti-ALPI polyclonal, supported by technical consulting from concept to clinic. Whether you need validated antibody pairs, optimized mono/polyclonal antibodies, or bulk ancillary reagents for intestinal disease research, we provide reliable supply solutions. Our global sourcing network ensures you can access even the most specialized targets without compromising on consistency or turnaround.