Anti-Alpha-synuclein Truncation 1-122 Monoclonal Antibody for Slot blot, ELISA - P37840

Core Product Specifications and Parameters

Biological Background: Alpha-synuclein Function and Localization

• Alpha-synuclein (also known as NACP, PARK1, PD1, PARK4) is a 14.46 kDa neuronal protein belonging to the synuclein family.
• It regulates synaptic vesicle trafficking and neurotransmitter release by acting as a monomer that enhances vesicle priming, fusion, and dilation of exocytotic fusion pores.
  Related references: PMID:28288128 PMID:30404828
• Functions as a molecular chaperone for SNARE proteins, ensuring proper folding and SNARE-complex assembly at the presynaptic membrane, a process vital for sustained synaptic function during aging.
  Related references: PMID:20798282
• Modulates dopamine neurotransmission by directly interacting with the dopamine transporter (DAT1) and influencing its activity and dopamine reuptake efficiency.
  Related references: PMID:26442590
• Subcellular localization includes the cytoplasm, membrane, nucleus, synapse, and axon; it is also secreted.
• Highly expressed in presynaptic terminals of the central nervous system, with principal expression in the brain.
• Associated with neurodegenerative disorders such as Parkinson disease and Alzheimer disease; features acetylation, phosphorylation, and ubiquitination post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds specifically to the N-terminal truncated form (aa 1-122) of human alpha-synuclein, lacking the C-terminal acidic tail. Consider this when interpreting results, as the antibody may not detect full-length alpha-synuclein or C-terminal epitopes.
• For slot blot applications, use appropriate protein immobilization and blocking steps to minimize nonspecific binding; optimize protein loading for clear detection.
• In ELISA, validate the antibody with recombinant 1-122 fragment or human brain tissue lysates and include controls for cross-reactivity with other synuclein family members (e.g., beta-synuclein, gamma-synuclein).
• As a rabbit monoclonal antibody, pair with anti-rabbit secondary detection reagents. Store at -20°C upon arrival (shipped on ice bag) to maintain stability.
• Application data are indicative; optimal working dilutions and conditions should be empirically determined in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing service for IVD raw materials, supplying this anti-alpha-synuclein monoclonal antibody along with validated antibody pairs and bulk ancillary reagents for neurodegenerative disease research. We support assay developers with optimized monoclonal and polyclonal antibodies, rare target sourcing, and technical consultation—covering every stage from concept to clinic. Whether you require custom antigen production or pre-validated panels for Parkinson's biomarker panels, CamelBio is your reliable supply partner.