Anti-Alpha-synuclein Truncation 1-119 Monoclonal Antibody [MJFF-C-24D2] for Slot blot, IF/ICC, ELISA - P37840

Core Product Specifications and Parameters

Biological Background: Alpha-synuclein Function and Localization

• Alpha-synuclein is a neuronal protein encoded by the SNCA gene, also known as NACP (Non-A beta component of AD amyloid) or PARK1, and is implicated in Parkinson's disease and other synucleinopathies.
• It plays a critical role in regulating synaptic vesicle trafficking and neurotransmitter release, particularly dopamine, thereby modulating synaptic activity. Related references: PMID:20798282 PMID:26442590 PMID:28288128
• As a monomer, alpha-synuclein enhances synaptic vesicle priming, fusion, and dilation of exocytotic fusion pores, a process dependent on increased local Ca²⁺ release from microdomains. Related references: PMID:28288128 PMID:30404828
• In its multimeric membrane-bound state, alpha-synuclein functions as a molecular chaperone, assisting in the folding of SNARE proteins at the presynaptic plasma membrane with cysteine string protein-alpha, which is crucial for maintaining SNARE-complex assembly during aging. Related references: PMID:20798282
• Subcellular localization includes the cytoplasm, membrane, nucleus, synapse, and extracellular secretion; the protein is also found in axons and presynaptic terminals.
• Highly and principally expressed in the brain, with enrichment in presynaptic terminals of the central nervous system.
• Post-translational modifications such as acetylation and phosphorylation occur; the protein is associated with amyloid formation and neurodegenerative disorders, including Parkinson's disease and Alzheimer's disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-119 amino acids, making this antibody specific to truncated alpha-synuclein forms; validate its reactivity with full-length alpha-synuclein (14 kDa) in your system if needed.
• For Slot blot applications, ensure adequate protein transfer and use an appropriate blocking agent to minimize background.
• In immunofluorescence (IF/ICC), optimize fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) to balance epitope preservation and accessibility.
• When performing ELISA, consider using recombinant alpha-synuclein 1-119 as a positive control and titrate antibody concentration for optimal signal-to-noise ratio.
• Cross-reactivity with human and mouse samples is reported; verify reactivity with other species if required.

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CamelBio empowers diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, from validated monoclonal antibodies like this anti-alpha-synuclein clone to ancillary reagents and rare target sourcing. Focusing on neurodegenerative disease research, CamelBio offers one-stop access to high-quality antibodies, bulk reagent supply, and tailored support to accelerate your assay development from concept to clinic.