Anti-Alpha-synuclein Monoclonal Antibody for WB, IF, IHC-P, ELISA - P37840

Core Product Specifications and Parameters

Biological Background: Alpha-synuclein Function and Localization

• Alpha-synuclein is a neuronal protein encoded by the SNCA gene (also known as NACP or PARK1) and is a member of the synuclein family.
• It plays critical roles in synaptic activity, including the regulation of synaptic vesicle trafficking and neurotransmitter release. Related references: PMID:20798282 PMID:26442590 PMID:28288128
• Functions as a monomer to enhance synaptic vesicle exocytosis by increasing local Ca2+ release from microdomains. Related references: PMID:30404828
• Acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of SNARE proteins to sustain SNARE-complex assembly during aging. Related references: PMID:20798282
• Associates with the dopamine transporter (DAT1) to modulate dopamine neurotransmission. Related references: PMID:26442590
• Subcellularly localized to the cytoplasm, membrane, nucleus, synapse, and is secreted; also found in cell projections including axons.
• Highly expressed in presynaptic terminals of the central nervous system, principally in brain.
• Post-translationally modified by acetylation, phosphorylation, and ubiquitylation; implicated in neurodegenerative diseases such as Parkinson's and Alzheimer's.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 59–140 of human alpha-synuclein, which includes the hydrophobic NAC domain; this region is conserved across human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blotting, consider using brain tissue lysates to detect the endogenous protein at ~14 kDa.
• For immunofluorescence or immunohistochemistry, take into account alpha-synuclein's multiple subcellular localizations (cytoplasm, membrane, nucleus, synapse, axon) and validate staining in relevant neuronal cell lines or brain sections.
• ELISA applications may benefit from using recombinant alpha-synuclein as a standard; ensure appropriate buffer conditions for monomeric or oligomeric forms as needed.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio serves as a comprehensive partner for IVD raw-material sourcing, offering an extensive portfolio of validated antibodies, antigens, and ancillary reagents for diagnostic assay development.
• With this anti-alpha-synuclein monoclonal antibody, researchers and manufacturers gain access to a key reagent for neurological disease biomarker studies, supported by CamelBio's capabilities in supplying bulk quantities, custom formulations, and rare-target raw materials.
• From concept to clinic, CamelBio streamlines procurement, technical consultation, and supply chain management, helping you accelerate your diagnostic projects.