Anti-Alpha SNAP Polyclonal Antibody for WB, IHC-P, ELISA - P54920

Core Product Specifications and Parameters

Biological Background: Alpha SNAP Function and Localization

• Alpha-soluble NSF attachment protein (Alpha SNAP) is encoded by the NAPA gene (also designated SNAPA), a 33 kDa member of the SNAP family of soluble NSF attachment proteins.
• It plays a critical role in mediating vesicular transport between the endoplasmic reticulum and the Golgi apparatus.
• In conjunction with the G protein subunit GNA12, Alpha SNAP facilitates the localization of CDH5 (VE-cadherin) to the plasma membrane, contributing to cell junction integrity. Related references: PMID:15980433
• Subcellular localization analyses indicate that Alpha SNAP is predominantly associated with the cell membrane.
• Post-translational modifications of Alpha SNAP include acetylation and phosphorylation, as identified by proteomics studies.
• It is part of the reference proteome for Homo sapiens and has been extensively identified in high-throughput proteomic screens.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal 295 amino acids of human Alpha SNAP, making this antibody likely to recognize both full‑length and N‑terminal fragments. Consider epitope accessibility when designing assays.
• For Western blot (WB) analysis, an expected band around 33 kDa can be anticipated under reducing conditions. Optimization of blocking and washing steps is advised.
• When performing immunohistochemistry on paraffin‑embedded sections (IHC‑P), antigen retrieval may be necessary; validate in the tissue and species of interest.
• In ELISA applications, the polyclonal nature may provide robust signal amplification. Pairing with a suitable detection system and verifying cross-reactivity in the intended sample matrix is recommended.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of Alpha SNAP antibodies and related reagents to support cellular transport and membrane trafficking research. As a dedicated IVD raw‑material partner, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our supply network can accommodate specialized needs, from common targets to rare analyte raw‑material sourcing, helping diagnostic manufacturers move efficiently from concept to clinic.