Anti-Alpha SNAP Monoclonal Antibody for WB and ELISA - P54920

Core Product Specifications and Parameters

Biological Background: Alpha SNAP Function and Localization

• Alpha-soluble NSF attachment protein (SNAPA), encoded by the NAPA gene, is a key component of the SNARE-mediated membrane fusion machinery.
• It facilitates vesicular transport between the endoplasmic reticulum and the Golgi apparatus, likely by promoting SNARE complex assembly and disassembly.
• Together with the G protein GNA12, Alpha SNAP directs the localization of cadherin 5 (CDH5) to the plasma membrane, contributing to cell–cell junction integrity. Related references: PMID:15980433
• Subcellularly, Alpha SNAP is primarily associated with the cell membrane, consistent with its role in membrane trafficking events.
• The protein undergoes post-translational modifications, including acetylation and phosphorylation, which may regulate its activity and interactions.
• Functional annotation highlights its involvement in ER-to-Golgi transport, protein transport, and overall membrane dynamics.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 201–295 of human Alpha SNAP, a region likely involved in SNARE interactions; this design provides high specificity for the target.
• For Western blot applications, use standard denaturing and reducing conditions; the expected molecular weight is ~33 kDa. Confirm band specificity with appropriate controls.
• In ELISA, optimize antigen coating concentrations and consider using blocking buffers compatible with monoclonal antibodies to reduce background.
• Given the antibody’s cross-reactivity with mouse and rat, validate performance in the relevant species’ sample matrix before use in quantitative assays.
• Pairing with appropriate detection reagents and secondary antibodies is recommended for consistent results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials. This Alpha SNAP Rabbit mAb addresses vesicular transport and membrane trafficking research needs, and is backed by CamelBio’s ability to supply validated antibody pairs, bulk ancillary reagents, and customized rare-target sourcing solutions. Whether integrating this antibody into existing ELISA or Western blot workflows or scaling up for commercial kit production, CamelBio provides end-to-end support from concept to clinic.