Anti-alpha Sarcoglycan (SGCA) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q16586

Core Product Specifications and Parameters

Biological Background: Alpha-Sarcoglycan Function and Localization

• Alpha-sarcoglycan (adhalin, 50DAG) is a 50 kDa type I transmembrane glycoprotein belonging to the sarcoglycan family; it contains a signal peptide, a large extracellular domain, a single transmembrane helix, and a short cytoplasmic tail.
• As a core component of the sarcoglycan subcomplex within the dystrophin-glycoprotein complex (DGC), it bridges the actin cytoskeleton to the extracellular matrix, crucial for maintaining sarcolemmal integrity during muscle contraction.
• Mutations in the SGCA gene cause autosomal recessive limb-girdle muscular dystrophy type 2D (LGMD2D), characterized by progressive muscle degeneration and weakness.
• Primarily localized to the sarcolemma (muscle cell membrane) and also detected in the cytoplasm in association with the cytoskeleton.
• Tissue distribution is limited: highest expression in skeletal muscle, lower in cardiac muscle, and trace amounts in lung, liver, and kidney; absent from brain.
• Post-translational modifications include N-glycosylation and phosphorylation; alternative splicing produces multiple isoforms (predicted by the signal and transmembrane features).
• Keywords from UniProt: Alternative splicing, Cell membrane, Cytoplasm, Cytoskeleton, Disease variant, Glycoprotein, Limb-girdle muscular dystrophy, Membrane, Phosphoprotein, Signal, Transmembrane.

Experimental Guidance and Technical Tips

• For Western blotting, alpha-sarcoglycan has a predicted molecular weight of approximately 43 kDa. Use reducing SDS-PAGE and validate detection in skeletal or cardiac muscle tissue lysates where expression is robust.
• The recombinant immunogen (aa 60-290) targets the extracellular domain; consider that epitope accessibility may differ between native and denatured conformations. Optimize sample preparation for your assay.
• In immunofluorescence/ICC, fixation with paraformaldehyde and mild permeabilization are recommended to preserve sarcolemmal localization. Include appropriate subcellular markers for co-localization studies.
• Cross-reactivity with mouse and rat makes this antibody useful for translational and preclinical studies; however, validate species-specific reactivity using control lysates from the respective species.
• For ELISA applications, ensure the coating antigen presents the immunogen region effectively. Titrate the antibody to determine optimal working concentrations for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive B2B supplier, connecting diagnostic manufacturers, research laboratories, and IVD developers with high-quality raw materials. For projects targeting alpha-sarcoglycan and related muscular dystrophy pathways, we can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our global procurement network excels at fulfilling rare-target and specialty raw material requests, streamlining your development from selection to supply.