Anti-alpha Sarcoglycan (SGCA) Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q16586

Core Product Specifications and Parameters

Biological Background: Alpha-Sarcoglycan Function and Localization

• Alpha-sarcoglycan (also known as 50DAG, adhalin, or dystroglycan-2) is a 50 kDa dystrophin-associated glycoprotein encoded by the SGCA gene in humans.
• It is an integral component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex (DGC) that forms a critical link between the F-actin cytoskeleton and the extracellular matrix.
• The sarcoglycan complex stabilizes the sarcolemma during muscle contraction/relaxation cycles and is essential for maintaining muscle fiber integrity.
• Subcellularly, alpha-sarcoglycan localizes primarily to the cell membrane, sarcolemma, and is also associated with the cytoplasm and cytoskeleton.
• Expression is highest in skeletal muscle, with lower levels in cardiac muscle and lung, and very weak expression in liver and kidney; it is absent from brain.
• Mutations in SGCA are associated with autosomal recessive limb-girdle muscular dystrophy type 2D (LGMD2D), characterized by progressive muscle weakness and wasting.
• Post-translational modifications include glycoprotein and phosphoprotein designations; the protein contains a signal peptide and a single transmembrane helix (UniProt keyword 'Transmembrane helix').

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 288-387 of human alpha-sarcoglycan (Q16586). This region is predicted to be in the extracellular domain, facilitating recognition in multiple applications.
• For Western blotting, expect a band at approximately 43 kDa in relevant tissue lysates (e.g., skeletal muscle). The antibody has reported cross-reactivity with mouse and rat; validation in these species is recommended.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), optimal staining conditions should be empirically determined using positive control tissues such as human skeletal muscle sections.
• For ELISA, consider using the synthetic peptide as a positive control and validate the antibody's specificity against full-length recombinant protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop sourcing partner for IVD raw materials, streamlining your path from concept to clinic. With this anti-alpha sarcoglycan antibody, researchers and diagnostic manufacturers targeting sarcolemma-associated disorders such as limb-girdle muscular dystrophies can access validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare or custom targets, CamelBio’s global supply network ensures reliable procurement of high-quality raw materials to accelerate your assay development.