Anti-Alpha Internexin Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q16352

Core Product Specifications and Parameters

Biological Background: Alpha Internexin Function and Localization

• Alpha-internexin (INA), also known as neurofilament 5 (NEF5), NF-66, or 66 kDa neurofilament protein, is a class-IV neuronal intermediate filament protein.
• It self-assembles and is involved in neuronal morphogenesis; it may form independent structural networks or cooperate with NEFL to create a filamentous backbone to which NEFM and NEFH attach.
• May also interact with the neuronal intermediate filament protein peripherin (PRPH) to form filamentous networks.
• Expression is predominantly found in the adult central nervous system (CNS).
• It is annotated with key post-translational modifications including acetylation and phosphorylation, and is classified as a glycoprotein.
• The protein contains a coiled-coil domain and plays roles in neurogenesis and neuronal differentiation.
• Alpha-internexin has been identified through direct protein sequencing and proteomics approaches.

Experimental Guidance and Technical Tips

• This antibody is raised against a synthetic peptide spanning amino acids 350-450 of human Alpha Internexin; peptide blocking experiments can be used to confirm specificity in your assay system.
• For Western blot, the predicted molecular weight is approximately 55 kDa; consider running appropriate positive controls from CNS tissue lysates.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), validation in human, mouse, or rat CNS tissues is recommended given the predominant expression in adult brain.
• Cross-reactivity with mouse and rat suggests utility in rodent model studies, but optimal working dilutions should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated monoclonal antibodies like this anti-Alpha Internexin antibody to support neuroscience and neurodegenerative disease research. Our integrated supply capabilities extend to bulk ancillary reagents and customized rare target sourcing, helping diagnostic manufacturers streamline development from concept to clinic.