Anti-Alpha Internexin Polyclonal Antibody for WB, ELISA - Q16352

Core Product Specifications and Parameters

Biological Background: Alpha-Internexin Function and Localization

• Alpha-internexin (also known as NF-66, NEF5) is a class-IV neuronal intermediate filament protein encoded by the INA gene in humans.
• It self-assembles and is involved in neuronal morphogenesis, potentially forming independent structural networks or cooperating with NEFL to provide a backbone for other neurofilament subunits.
• May also cooperate with peripherin (PRPH) to form filamentous networks, contributing to neuronal cytoskeletal organization.
• Predominantly expressed in the adult central nervous system.
• Subject to acetylation and phosphorylation, as indicated by proteomics identification.
• The protein has a molecular weight of approximately 55 kDa (calculated 55,391 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 13-300 of human alpha-internexin, a region containing the coiled-coil rod domain; this design may confer good specificity for the target.
• For Western blotting, a protein band of approximately 55 kDa is anticipated; ensure proper sample preparation from neural tissues or cell lines expressing alpha-internexin.
• In ELISA applications, consider direct or sandwich formats; preliminary checkerboard titrations are recommended to determine optimal antibody and antigen concentrations.
• Cross-reactivity with mouse alpha-internexin is reported, making this antibody suitable for studies in mouse models; sequence alignment suggests potential reactivity with other species, but validation is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies for neuroscience research. This alpha-internexin antibody supports diagnostic and research workflows; we also supply matched antibody pairs, bulk reagents, and custom sourcing for rare targets. Partner with CamelBio to streamline your raw-material procurement from concept to clinical application.