Anti-Alpha Fodrin Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13813

Core Product Specifications and Parameters

Biological Background: Alpha Fodrin (SPTAN1) Function and Localization

• SPTAN1 encodes spectrin alpha chain, non-erythrocytic 1 (Alpha-II spectrin/fodrin alpha chain), a core component of the non-erythroid spectrin-based membrane skeleton.
• Alternative splicing generates multiple isoforms, which may contribute to distinct functional properties in different tissues or developmental stages.
• Fodrin interacts with calmodulin in a calcium-dependent manner, linking calcium signaling to cytoskeletal reorganization.
• This protein is implicated in secretion, likely through its role in calcium-dependent movement of the cytoskeleton at the plasma membrane.
• Subcellular localization is primarily at the cytoplasm, cytoskeleton, and cell cortex, consistent with its role as a membrane-associated scaffold.
• Key structural features include actin-binding domains, spectrin repeats, and an SH3 domain, which mediate protein-protein interactions and network assembly.
• Clinically, variants in SPTAN1 are associated with neurological disorders, including developmental and epileptic encephalopathy (DEE5), early infantile epileptic encephalopathy (EIEE5), hereditary spastic paraplegia, and intellectual disability.

Experimental Guidance and Technical Tips

• For western blotting, a low-percentage polyacrylamide gel (e.g., 6%) and PVDF membrane with extended transfer time are recommended to resolve the high molecular weight (285 kDa) spectrin protein.
• Consider using a positive control lysate from human, mouse, or rat brain or epithelial tissues, where fodrin is abundant.
• Since this is a polyclonal antibody, lot-to-lot consistency should be verified by the end user in their specific assay format and sample type.
• For immunofluorescence, permeabilization with Triton X-100 or digitonin may be required to access cytoskeletal and cortical antigens.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined, single-source solution for diagnostic manufacturers and research labs seeking high-quality IVD raw materials. From validated antibody pairs and monoclonal/polyclonal antibodies to bulk ancillary reagents and custom rare-target sourcing, we cover the full development pipeline. This Alpha Fodrin antibody fits into neuroscience and cell biology reagent panels, and our team can support your broader procurement needs with efficient supply chain management.