Anti-Alpha-Fetoprotein (AFP) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P02771

Core Product Specifications and Parameters

Biological Background: Alpha-Fetoprotein (AFP) Function and Localization

• Alpha-fetoprotein (AFP), also known as alpha-1-fetoprotein and alpha-fetoglobulin, is encoded by the AFP gene in humans.
• It is a secreted glycoprotein that binds copper, nickel, and fatty acids, and to a lesser extent, bilirubin, with similarity to serum albumin.
• Only a small fraction (<2%) of human AFP exhibits estrogen-binding properties.
• AFP is predominantly synthesized by the fetal liver and yolk sac, and is found in plasma.
• Post-translational modifications include glycosylation, disulfide bond formation, phosphorylation, and sulfation, as indicated by UniProt keywords.
• Structurally, AFP contains repeated domains and multiple disulfide bonds, contributing to its stability and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 360–609 of human AFP, covering the C-terminal domain; consider this region when designing detection strategies.
• For Western blot, denaturing and reducing conditions are recommended; a specific band around 69 kDa is expected.
• In immunofluorescence and ELISA, validate the antibody in the relevant sample system and optimize fixation/permeabilization conditions as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined gateway to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this Anti-AFP pAb, and bulk ancillary reagents for diagnostic development. Whether you are working on cancer biomarker detection or fetal health diagnostics, our supply chain ensures consistent quality and access to rare-target raw materials.