Anti-Alpha-Fetoprotein (AFP) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P02771

Core Product Specifications and Parameters

Biological Background: Alpha-Fetoprotein (AFP) Function and Localization

• Alpha-fetoprotein (AFP), also referred to as alpha-1-fetoprotein or alpha-fetoglobulin, is encoded by the human AFP gene.
• AFP belongs to the albumin gene family and shares structural features with serum albumin.
• This secreted protein is synthesized by the fetal liver and yolk sac, and circulates in fetal plasma.
• AFP functions as a carrier protein, binding copper, nickel, fatty acids, and, to a lesser extent, bilirubin. A small proportion (<2%) interacts with estrogen.
• Post-translational modifications include glycosylation, phosphorylation, disulfide bond formation, and sulfation; the protein's three-dimensional structure has been solved.
• Due to its fetal origin and secretion into plasma, AFP levels are developmentally regulated.

Experimental Guidance and Technical Tips

• The immunogen for this antibody corresponds to amino acids 395-609 of the human AFP sequence. Consider this region when designing epitope mapping or blocking experiments.
• For Western blot (WB), a band around 69kDa is expected; include appropriate positive and negative controls for your specific sample type.
• For immunofluorescence (IF/ICC), optimal fixation and permeabilization conditions should be determined empirically for your cell line or tissue.
• For ELISA, pair this monoclonal antibody with a suitable detection antibody and validate using recombinant AFP or reference standards.
• Cross-reactivity is reported for human AFP; verify species specificity if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and labs. This Alpha-Fetoprotein (AFP) antibody is part of our comprehensive portfolio for biomarker research and assay development. Our expert team provides tailored technical and supply chain support, helping you bridge the gap from early-stage R&D to clinical applications.