Anti-Alpha-Fetoprotein (AFP) Monoclonal Antibody for WB, IF/ICC, ELISA - P02771

Core Product Specifications and Parameters

Biological Background: Alpha-Fetoprotein Function and Localization

• Alpha-fetoprotein (AFP) is a major fetal plasma protein, structurally related to albumin, and functions as a transport molecule for various ligands including fatty acids, bilirubin, and metal ions such as copper and nickel.
• AFP is predominantly synthesized by the fetal liver and yolk sac, and is secreted into the fetal circulation; after birth, its levels rapidly decline, making it a useful tumor marker in adults.
• It belongs to the albuminoid gene family and shares multiple disulfide-bonded repeat domains, contributing to its carrier properties.
• Post-translational modifications include glycosylation, phosphorylation, and sulfation, which may influence its stability and function.
• AFP has a weaker affinity for bilirubin compared to serum albumin, and less than 2% of human AFP exhibits estrogen-binding activity.
• As a secreted glycoprotein, AFP is readily detectable in serum and other biological fluids, supporting its application in immunoassays for hepatocellular carcinoma and germ cell tumors.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is ~69 kDa; note that glycosylation may cause the observed band to shift slightly higher. Validate detection under reducing and non-reducing conditions.
• When performing IF/ICC, consider that AFP is a secreted protein; intracellular staining may be enhanced by using a protein transport inhibitor (e.g., brefeldin A) to accumulate AFP in the Golgi.
• For ELISA applications, paired with a suitable detection antibody, ensure thorough validation with recombinant protein standards and appropriate biological matrix controls.
• This rabbit monoclonal antibody was generated using a near full-length recombinant immunogen (aa 19-609), which may provide broad epitope coverage; nonetheless, confirm specificity in your sample system using appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with streamlined access to premium IVD raw materials. For liver cancer biomarker studies and AFP-based immunoassay development, we supply validated antibody pairs, optimized recombinant monoclonal antibodies like this anti-AFP clone, bulk ancillary reagents, and customized sourcing for rare-target raw materials. Rely on CamelBio to accelerate your development pipeline from concept to clinical readiness.