Anti-Alpha-Fetoprotein (AFP) Monoclonal Antibody for WB, ELISA - P02771

Core Product Specifications and Parameters

Biological Background: Alpha-Fetoprotein Function and Localization

• Alpha-fetoprotein (AFP) is a secreted plasma protein that functions as a carrier for metal ions, fatty acids, and bilirubin.
• It binds copper and nickel, and to a lesser extent, bilirubin, when compared to serum albumin.
• AFP is primarily synthesized by the fetal liver and yolk sac, and its plasma levels decline after birth.
• A minor fraction of human AFP (<2%) exhibits estrogen-binding capacity.
• The protein undergoes post-translational modifications including glycosylation, phosphorylation, and sulfation.
• It contains disulfide bonds and structural repeats, contributing to its stable secreted conformation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length mature AFP (residues 19-609), which likely generates antibodies recognizing both native and denatured forms; consider this for your assay design.
• For WB, the predicted molecular weight is ~69 kDa; verify the band in your specific sample lysate, as glycosylation may cause slight size shifts.
• Since AFP is a secreted protein, ensure sample preparation (e.g., serum, cell supernatant) is appropriate for detection.
• Validate antibody specificity using appropriate positive (e.g., HepG2 cell lysate, human serum) and negative controls.
• For ELISA, pairing with a capture antibody may be optimized; test in sandwich ELISA format using recombinant AFP standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your raw-material procurement for IVD development, offering a comprehensive portfolio including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. As a one-stop sourcing partner, we can support your Alpha-Fetoprotein (AFP) immunoassay projects from concept to clinic, ensuring you receive consistent, high-quality materials. Contact our team to discuss your specific AFP antibody requirements, bulk orders, or custom sourcing needs.