Anti-Alpha-2-Macroglobulin (A2M) Polyclonal Antibody for WB - P01023

Core Product Specifications and Parameters

Biological Background: Alpha-2-Macroglobulin (A2M) Function and Localization

• Alpha-2-macroglobulin (A2M) is a large plasma glycoprotein and member of the alpha-macroglobulin family, also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 (CPAMD5).
• A2M acts as a broad-spectrum protease inhibitor, capable of inhibiting all four classes of proteinases (serine, cysteine, aspartic, and metalloproteinases) through a unique 'trapping' mechanism.
• The protein contains a 'bait region' with specific cleavage sites; upon proteolytic cleavage, a conformational change traps the proteinase, and a thioester bond mediates covalent binding to the enzyme.
• Following cleavage, the entrapped proteinase remains active against low molecular weight substrates but shows greatly reduced activity against high molecular weight substrates.
• A2M is secreted into the blood plasma and functions as a key extracellular protease scavenger, contributing to the regulation of coagulation, fibrinolysis, and inflammation.
• The protein contains multiple structural features, including a signal peptide, disulfide bonds, glycosylation sites, and an internal thioester bond; its mature molecular weight is approximately 163 kDa.
• Three-dimensional structural studies have elucidated the conformational changes underlying the trapping mechanism.

Experimental Guidance and Technical Tips

• The immunogen was designed against a recombinant fragment corresponding to amino acids 1155–1474, which includes the C-terminal receptor-binding domain; consider this when assessing antibody reactivity with different A2M conformations or fragments.
• For Western blot (WB), a band at ~163 kDa under reducing or non-reducing conditions is expected; validate detection in the relevant sample system and consider pre-adsorption for high specificity.
• For immunohistochemistry (IHC-P), appropriate antigen retrieval methods and tissue fixation conditions should be optimized experimentally.
• For ELISA, the antibody may be used as capture or detection reagent; pairing with another clone or conjugate should be validated in the specific assay format.
• Cross-reactivity with other alpha-macroglobulin family members (e.g., pregnancy zone protein) has not been tested; confirm specificity if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to high-quality IVD raw materials, including this rabbit anti-A2M polyclonal antibody, ideal for studying protease inhibition and plasma protein dynamics. Our supply capabilities cover validated antibody pairs, bulk ancillary reagents, and rare-target sourcing, helping you accelerate diagnostic development projects from concept to clinic.