Anti-alpha 1 Spectrin Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P02549

Core Product Specifications and Parameters

Biological Background: alpha 1 Spectrin Function and Localization

• Spectrin alpha chain, erythrocytic 1 (SPTA1) is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane.
• It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane, maintaining cell shape and stability.
• SPTA1 is localized to the cytoplasm, cytoskeleton, and cell cortex, reflecting its role in membrane-cytoskeleton interactions.
• The protein contains multiple spectrin repeats, an SH3 domain, and a calcium-binding EF-hand motif, which are critical for its structural and signaling functions.
• Alternative splicing produces multiple isoforms with tissue-specific functions, expanding the functional diversity of spectrin.
• Mutations in SPTA1 are linked to hereditary elliptocytosis, pyropoikilocytosis, and hemolytic anemia, underscoring its clinical relevance.
• Phosphorylation and proteomics studies have identified SPTA1 as a key node in erythrocyte biology and membrane mechanics.
• The molecular weight is approximately 280 kDa, as derived from sequence and confirmed by SDS-PAGE analysis.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 940-1160) covers a central repeat region, which may recognize multiple SPTA1 isoforms; validate specificity in your experimental system.
• For Western blot, the predicted molecular weight is ~280 kDa; use low-percentage (e.g., 6%) polyacrylamide gels and wet transfer conditions optimized for high molecular weight proteins.
• In immunofluorescence (IF) and immunohistochemistry (IHC), optimize fixation (e.g., 4% PFA) and permeabilization to preserve the erythrocyte membrane cytoskeleton; antigen retrieval may enhance epitope accessibility.
• For ELISA, consider coating with the recombinant immunogen or erythrocyte membrane extracts, and titrate the antibody to determine optimal working concentration.
• Cross-reactivity with mouse and rat SPTA1 has been reported; perform species-specific validation if using samples from these organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined, one-stop access to premium IVD raw materials and technical support. This anti-alpha 1 Spectrin antibody supports the development of assays targeting erythrocyte disorders and cytoskeletal integrity. From validated antibody pairs to bulk ancillary reagents and rare target sourcing, CamelBio accelerates your project from concept to clinic with reliable supply capabilities.