Anti-Alpha-1 Antitrypsin (SERPINA1) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P01009

Core Product Specifications and Parameters

Biological Background: Alpha-1 Antitrypsin Function and Localization

• Alpha-1 antitrypsin (SERPINA1) is a major serine protease inhibitor (serpin) that primarily targets elastase, with moderate affinity for plasmin and thrombin.
• Its key physiological role is protecting the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE), thereby preserving lung tissue integrity.
• It irreversibly inhibits trypsin, chymotrypsin, and plasminogen activator; aberrant forms may exhibit altered functions, such as proteolytic activity against insulin and plasmin.
• The protein is secreted and found in the extracellular space and extracellular matrix, but can also localize to the endoplasmic reticulum.
• SERPINA1 is ubiquitously expressed, with notable presence in leukocytes and plasma.
• It undergoes alternative splicing and post-translational modifications, including glycosylation and phosphorylation, and is classified as an acute-phase protein.
• Additional keywords: blood coagulation, hemostasis, signal peptide, and direct protein sequencing evidence.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 156–255) targets a specific region of SERPINA1; the antibody may recognize both native and recombinant protein.
• For Western blotting, a band around 47 kDa is expected; reducing conditions are typically suitable.
• This polyclonal antibody has been validated for WB, IHC-P, IF/ICC, and ELISA. Consider including appropriate positive and negative controls.
• Cross-reactivity with mouse has been reported; validate in the relevant sample system if mouse models are used.
• Optimization of antibody dilution and incubation conditions is recommended for each application and experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner for diagnostic manufacturers and research laboratories, offering a streamlined supply chain from concept to clinic. This Alpha-1 Antitrypsin (SERPINA1) antibody, targeting a critical protease inhibitor in respiratory and inflammatory research, exemplifies our capability to provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. We support your projects with rigorous quality and one-stop convenience without claiming service guarantees beyond material supply.