Anti-Alpha-1 Antitrypsin (SERPINA1) Rabbit Monoclonal Antibody for WB, ELISA - P01009

Core Product Specifications and Parameters

Biological Background: Alpha-1 Antitrypsin Function and Localization

• Alpha-1-antitrypsin (also known as SERPINA1, AAT, PI) is a member of the serpin superfamily of serine protease inhibitors.
• Its primary physiological role is to inhibit neutrophil elastase, protecting the lower respiratory tract from proteolytic degradation; it also inhibits trypsin, chymotrypsin, and plasminogen activator.
• An aberrant form can inhibit insulin-induced nitric oxide synthesis and exhibits proteolytic activity against insulin and plasmin.
• The protein is secreted into the extracellular space and is also found in the endoplasmic reticulum and extracellular matrix.
• It is ubiquitously expressed, notably in leukocytes and plasma, consistent with its role as an acute-phase protein and involvement in hemostasis.
• Post-translational modifications include glycosylation and phosphorylation, which may influence stability and function.

Experimental Guidance and Technical Tips

• The immunogen covers full-length human Alpha-1 Antitrypsin (amino acids 1-418), so the antibody may detect both native and denatured conformations; validation in the relevant assay setup is advised.
• For Western blot, expect a band near 47 kDa; optimize gel percentage and transfer parameters accordingly.
• Reported cross-reactivity with mouse and rat should be confirmed with species-specific positive and negative controls.
• When using ELISA, consider performing a checkerboard titration of coating antigen and antibody to determine optimal conditions.
• Upon receipt, store at the recommended temperature to maintain antibody integrity (shipped on ice bags).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For Alpha-1-antitrypsin (SERPINA1) research—such as protease inhibitor studies or respiratory disease biomarker development—we offer reliable monoclonal antibody options and can assist in sourcing rare target raw materials to streamline your assay development pipeline.