Anti-Alpha-1 Antichymotrypsin (SERPINA3) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P01011

Core Product Specifications and Parameters

Biological Background: Alpha-1-antichymotrypsin Function and Localization

• Alpha-1-antichymotrypsin, encoded by the SERPINA3 gene, belongs to the serpin superfamily of serine protease inhibitors.
• It inhibits neutrophil cathepsin G and mast cell chymase, enzymes capable of converting angiotensin-1 to the active angiotensin-2.
• Also known as cell growth-inhibiting gene 24/25 protein, it may modulate cellular proliferation.
• The protein is secreted and circulates in plasma, with predominant synthesis in the liver.
• As an acute-phase reactant, its concentration increases markedly during inflammation or infection.
• It is a glycoprotein with a signal peptide, consistent with its secretory pathway; alternative splicing produces multiple isoforms.
• The canonical sequence has a calculated molecular mass of approximately 47.7 kDa, though glycosylation increases the apparent weight.
• It is detected in the amyloid plaques of Alzheimer disease hippocampus, suggesting a role in neurodegenerative pathology.

Experimental Guidance and Technical Tips

• The immunogen (residues 24-423) covers the mature protein; it may detect both full-length and processed forms if present in samples.
• For Western blot, the predicted band is around 48 kDa; glycosylation can cause slight mobility shifts.
• In IHC-P, adequate antigen retrieval and permeabilization may be required to access the secreted target in tissue sections.
• When developing sandwich ELISAs, consider pair this antibody with a capture reagent recognizing a distinct epitope.
• Sample collection timing is important for acute-phase targets; levels may fluctuate with inflammatory status.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with seamless access to high-quality IVD raw materials. For Alpha-1-antichymotrypsin (SERPINA3) studies, we supply validated polyclonal antibodies like this rabbit pAb, along with complementary monoclonal antibodies, recombinant proteins, and bulk ancillary reagents. Our sourcing network can procure rare-target materials to support your diagnostic development from concept to clinic.