Anti-Alpha-1 Antichymotrypsin (SERPINA3) Rabbit Monoclonal Antibody for WB, ELISA - P01011

Core Product Specifications and Parameters

Biological Background: Alpha-1 Antichymotrypsin Function and Localization

• Alpha-1-antichymotrypsin (SERPINA3, also known as ACT, AACT, GIG24, GIG25) is a member of the serpin (serine protease inhibitor) superfamily.
• The protein functions as a protease inhibitor, targeting neutrophil cathepsin G and mast cell chymase, which can convert angiotensin-1 to the active angiotensin-2.
• It is a secreted glycoprotein with a signal peptide, undergoing post-translational glycosylation.
• Synthesis primarily occurs in the liver, and the protein is found circulating in plasma; its levels increase during the acute phase of inflammation or infection.
• SERPINA3 has been detected in amyloid plaques from the hippocampus of Alzheimer disease brains, suggesting a role in neuroinflammation or plaque formation.
• Alternative splicing is reported, which may result in multiple protein isoforms.
• Structural and proteomics studies have been conducted, as indicated by keywords: 3D-structure, Direct protein sequencing, and Proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant fusion protein covering the full-length human SERPINA3 sequence (aa 1-423); this design may favor antibodies that recognize native and denatured forms, suitable for both WB and ELISA.
• For Western blot (WB), the predicted molecular weight of target protein is approximately 48 kDa (theoretical mass ~47.7 kDa), so ensure adequate separation in that range.
• ELISA applications may be developed; consider using this monoclonal antibody as a capture or detection reagent in sandwich or indirect formats, with validation of binding linearity and specificity.
• Cross-reactivity is reported only for human; for studies in other species, ortholog conservation should be examined, and cross-reactivity may need empirical testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive sourcing solution for IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For Alpha-1 Antichymotrypsin (SERPINA3), an acute-phase and Alzheimer's-associated biomarker, our portfolio includes this rabbit monoclonal antibody and access to customized reagent sourcing to support your assay development and clinical research needs.