Anti-ALOXE3 Polyclonal Antibody for WB, ELISA - Q9BYJ1

Core Product Specifications and Parameters

Biological Background: ALOXE3 Function and Localization

• ALOXE3 is a non-heme iron-containing lipoxygenase that functions primarily as a hydroperoxide isomerase, converting fatty acid hydroperoxides into epoxyalcohols and ketones. Related references: PMID:12881489, PMID:17045234, PMID:20923767
• In the skin, it processes linoleate in esterified omega-hydroxyacyl-sphingosine ceramides, generating an epoxy-ketone derivative essential for covalent attachment to membrane proteins, thus forming the corneocyte lipid envelope and establishing the skin water barrier. Related references: PMID:21558561
• ALOXE3 also exhibits residual lipoxygenase activity, oxygenating polyunsaturated fatty acids like arachidonic acid to produce hydroperoxides. Related references: PMID:20921226
• Through hepoxilin production, it may contribute to adipocyte differentiation by activating PPARG and regulate inflammatory pain signaling in the spinal cord.
• The protein is predominantly expressed in the skin and localizes to the cytoplasm.
• Keywords including alternative splicing suggest multiple isoforms, and mutations in ALOXE3 are linked to ichthyosis, highlighting its role in epidermal homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1–280 amino acids; consider its coverage of known isoforms when interpreting results.
• For Western blotting, validate antibody performance in skin tissue lysates from human, mouse, or rat, given the skin-specific expression profile.
• For ELISA, optimize coating and detection conditions using purified recombinant protein or tissue extracts.
• As a cytoplasmic protein, prepare whole-cell or cytoplasmic fractions; avoid nuclear-enriched preparations.
• Polyclonal antibodies may exhibit lot-to-lot variability; include appropriate positive and negative controls in each experiment.
• Cross-reactivity with mouse and rat makes this antibody suitable for translational studies in rodent models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with a one-stop supply of validated IVD raw materials, including optimized antibodies for skin biology and lipid metabolism targets. We offer comprehensive procurement solutions—from monoclonal/polyclonal antibodies and antibody pairs to bulk ancillary reagents—and can source rare raw materials to accelerate your assay development from concept to clinic.