Anti-ALOX5 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P09917

Core Product Specifications and Parameters

Biological Background: ALOX5 Function and Localization

• ALOX5 encodes polyunsaturated fatty acid 5-lipoxygenase (also known as arachidonate 5-lipoxygenase, 5-LO, 5-lipoxygenase), a key enzyme in the biosynthesis of leukotrienes, potent inflammatory mediators, via oxygenation of arachidonic acid. Related references: PMID:19022417, PMID:21233389, PMID:22516296
• It converts arachidonate to 5-hydroperoxyeicosatetraenoate (5-HPETE) and then to leukotriene A4 (LTA4), and can also produce 8-HPETE and 12-HPETE. Related references: PMID:23246375
• ALOX5 displays lipoxin synthase activity, converting (15S)-HETE into a conjugate tetraene, and metabolizes other polyunsaturated fatty acids to form specialized pro-resolving mediators (SPMs) like lipoxins and resolvins, contributing to anti-inflammatory responses. Related references: PMID:17114001, PMID:21206090, PMID:31664810
• May also participate in dendritic cell migration, wound healing, monocyte adhesion, and adaptive humoral immunity; directly interacts with CD40 via PIK3R1 to produce ROS in B cells. Related references: PMID:21200133
• It has a role in glucose homeostasis, insulin secretion regulation, and palmitic acid-induced insulin resistance via AMPK, and influences bone mineralization and fat cell differentiation.
• Subcellularly, ALOX5 is found in cytoplasm, nucleus matrix and membrane, perinuclear region, cytosol, nuclear envelope, and intermembrane space.
• It is a calcium- and iron-binding enzyme, classified as a dioxygenase and hydrolase, with alternative splicing and phosphorylation modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–200) of human ALOX5; the antibody may detect full-length and possibly N-terminal fragments. Validate specificity in your sample system.
• Predicted band size is ~78 kDa in Western blot; consider using appropriate molecular weight markers and loading controls. Optimize lysis buffers and protease inhibitors for membrane and nuclear fractions.
• For immunohistochemistry (IHC‑P), test antigen retrieval methods (citrate or EDTA buffer) and antibody titration. Include relevant positive and negative tissue controls.
• For immunofluorescence (IF/ICC), permeabilization with Triton X‑100 may be necessary to access nuclear and cytoplasmic pools.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays. Consider peptide blocking or siRNA knockdown to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop supply channel for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-ALOX5 antibody supports research in leukotriene signaling, inflammation, and immunology. For rare-target raw material sourcing or custom reagent development, our team can bridge gaps from discovery to diagnostic application.