Anti-ALOX5 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P09917

Core Product Specifications and Parameters

Biological Background: ALOX5 Function and Localization

• ALOX5 (5-lipoxygenase, 5-LO) catalyzes the initial steps of leukotriene biosynthesis by oxygenating arachidonate to 5-HPETE and then dehydrating it to LTA4, potent inflammatory mediators. Related references: PMID:19022417 PMID:21233389 PMID:22516296

• Additionally, ALOX5 oxygenates arachidonate to 8-HPETE and 12-HPETE. Related references: PMID:23246375

• It functions as a lipoxin synthase, converting (15S)-HETE into a conjugate tetraene. Related references: PMID:31664810

• ALOX5 metabolizes eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) into specialized pro-resolving mediators (SPMs) such as resolvins E and D, contributing to the resolution of inflammation. Related references: PMID:17114001 PMID:21206090 PMID:31664810

• The enzyme localizes to the cytoplasm, nucleus matrix, nuclear membrane, perinuclear region, cytosol, and nuclear envelope.

• ALOX5 participates in adaptive immunity by regulating B cell and follicular helper T cell functions and mediates CD40-induced ROS production in B cells via PIK3R1 bridging. Related references: PMID:21200133

• It is a dioxygenase, hydrolase, and iron-binding protein belonging to the leukotriene biosynthesis pathway.

• By similarity, ALOX5 may play a role in dendritic cell migration, wound healing via heme oxygenase-1 regulation, monocyte adhesion, glucose homeostasis, insulin secretion, bone mineralization, and fat cell differentiation.

Experimental Guidance and Technical Tips

• For western blot (WB), consider using whole cell lysates from human, mouse, or rat tissues/cells known to express ALOX5.
• Validate antibody specificity by using a blocking peptide or knockdown/knockout controls in the relevant sample system.
• For immunohistochemistry (IHC-P), test antigen retrieval conditions and antibody dilution; use tissues with known ALOX5 expression as positive controls.
• In immunofluorescence (IF/ICC), optimize fixation and permeabilization to preserve both cytoplasmic and nuclear localization signals.
• For ELISA, this antibody may be used as capture or detection reagent after pairing optimization.
• The immunogen sequence corresponds to an internal region (aa 100–200), so the antibody may recognize both native and recombinant ALOX5.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, supporting every stage from concept to clinic. Our ALOX5 antibody is part of a comprehensive portfolio for inflammation and immune research, backed by validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. We also specialize in sourcing rare target raw materials, ensuring you get the right tools for your assay development and biomarker studies.