Anti-ALOX5 Monoclonal Antibody for WB, IF-P, ELISA - P09917

Core Product Specifications and Parameters

Biological Background: ALOX5 Function and Localization

• ALOX5 encodes polyunsaturated fatty acid 5-lipoxygenase (5-LO, 5-lipoxygenase), a member of the lipoxygenase family. Multiple isoforms exist, producing bands at 78 kDa (predicted), 71 kDa, 74 kDa, 61 kDa, and 56 kDa in various tissues.
• Catalyzes the first two steps in leukotriene biosynthesis: oxygenation of arachidonate to 5-HPETE and subsequent dehydration to LTA4, a key mediator of inflammation. Related references: PMID:19022417, PMID:21233389, PMID:22516296
• Also converts arachidonate into 8-HPETE and 12-HPETE, demonstrating broader substrate flexibility. Related references: PMID:23246375
• Exhibits lipoxin synthase activity by converting (15S)-HETE into a conjugate tetraene, participating in the resolution of inflammation. Related references: PMID:31664810
• Metabolizes oxidized fatty acids (EPA and DHA) to specialized pro-resolving mediators (resolvins D and E), contributing to anti-inflammatory responses. Related references: PMID:17114001, PMID:21206090, PMID:31664810
• Participates in adaptive immunity by regulating B cell responses and CD40-induced ROS production via interaction with PIK3R1. Related references: PMID:21200133
• Subcellular localization includes cytoplasm, nuclear matrix, nuclear membrane, perinuclear region, and cytosol, reflecting its dynamic role in signal transduction. Keywords indicate it is a calcium- and iron-binding dioxygenase, oxidoreductase, and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100) of human ALOX5. For WB, whole-cell lysates from human, mouse, or rat tissues/cell lines known to express ALOX5 are recommended. Expect a predominant band near 78 kDa; additional bands at lower molecular weights may represent isoforms or fragments.
• For IF-P, it is advisable to include appropriate controls (e.g., isotype, peptide competition, or knockout/knockdown samples) to confirm specificity.
• In ELISA applications, verify that the capture/detection format does not interfere with the epitope recognized by this antibody, particularly if using peptide-based assays.
• Storage: Upon arrival, store at -20°C. Avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner for IVD raw materials, supporting the entire development pipeline from concept to clinic. The ALOX5 Rabbit mAb (5-LO) is ideal for leukotriene biosynthesis and inflammation-related research, and CamelBio can further supply validated antibody pairs, bulk ancillary reagents, and custom rare-target raw material procurement to accelerate your diagnostic assay development.